Current Pharmaceutical Analysis - Volume 20, Issue 8, 2024

BackgroundAlzheimer's disease (AD) impacts a total of 30 million individuals worldwide, resulting in the impairment of brain cells. Despite the approval of medications, there are still poor results and adverse effects that continue to exist, which has led to continued research for more effective treatments.ObjectivesIn this study prioritize the medicinal plants and their bioactive chemicals to attract researchers and phar Read More

BackgroundAnticancer herbal drugs have gained significant attention in pharmaceutical research due to their complex chemical profiles and multifaceted therapeutic effects. Electrochemical analysis has emerged as a powerful tool for studying these compounds, offering unique insights into their behavior and properties.MethodsThis review examines recent advances in the electrochemical analysis of five key anticancer Read More

BackgroundAnalytical methods have an essential function in the healthcare sector, particularly when it emanates from the creation and approval of medications. These methods are essential for identifying the active ingredients, ensuring the purity of compounds, and quantifying the existence of any impurities or degradation products.ObjectiveThe objective of the research article is to discuss an analytical method available for the Read More

Biotechnology, a field discovered in 1919, unites biology and engineering to harness living organisms for medical purposes. Fueled by using DNA's discovery in the 1950s, biotechnology has converted through genetic engineering, yielding impactful merchandise regulated by means of entities like the FDA. The manufacturing involves upstream and downstream processing including the various techniques involved in the downstr Read More

ObjectiveThe aim of the study was to establish a GC-MS method for the determination of extractable substances in the stoppers of Ceftriaxone sodium bottles for injection, to establish an HPLC method for the determination of vulcanizing agent and antioxidant in Ceftriaxone sodium for injection bottle stopper and to analyze the correlation between the volatile substances of the rubber stopper and the clarity of solution. Read More

BackgroundRadix astragali (a Tonic-Qi traditional Chinese medicine) can enhance cellular metabolism and increase the lifespan of cells. Spermidine can markedly extend the lifespan. On the basis of these considerations, we assumed whether any relationship existed between the anti-aging activity of Radix astragali and spermidine.MethodsIn this research, spermidine and 10 other kinds of biogenic amines in Radix astragali s Read More

BackgroundHispidulin, a flavonoid renowned for its diverse pharmacological activities, is abundant in S. barbata D. Don. Given its prevalence, it is essential to establish a dependable analytical method for quantifying hispidulin in this plant.MethodsThis study introduces a sensitive and reliable high-performance thin-layer chromatographic (HPTLC) method for both qualitative and quantitative analysis of hispidulin in S. barbata D Read More

BackgroundVitamin A and D deficiency in children is a common public health problem. In China, almost all children are administered vitamin A and D preparations daily and they should be consumed continuously until the age of 18. Children are sensitive to elemental impurities, including heavy metals. However, there are no regulatory requirements or reports on the risk assessment of elemental impurities in marketed vitamin A Read More

BackgroundAtypical peaks were observed in capillary electrophoresis with sodium dodecyl sulfate (CE-SDS) during the development of therapeutic monoclonal IgG4 antibodies (mAb-X). Based on the previous literature reports, the atypical peak may be caused by various factors such as post-translational modifications (PTMs), method-induced artifacts, sample degradation and sequence variants. Due to the high complexity str Read More

BackgroundPurified human albumin fractionated from plasma has a complex matrix that imposes a lot of interference in quality control testing, particularly for impurities at trace level. In this study, a simple approach has been studied and validated for the quantification of aluminium content using Graphite Furnace Atomic Absorption Spectrometry (GF-AAS). As per international guidelines, the linearity, accuracy, precision, spe Read More

BackgroundConventional chromatographic techniques and direct analysis pose challenges in detecting certain potential genotoxic impurities, primarily due to factors such as the absence of a chromophore, low molecular weight, and insufficient chromatographic retention caused by high polarity. One such compound, N, O-Dimethyl Hydroxylamine, is of particular concern due to its structural similarity to Hydroxylamine, a r Read More

BackgroundAqueous solubility is a key parameter in understanding drug transport in the body and also in the development of analytical methods. Determination of a complete pH-solubility profile is essential during the pre-formulation stage, and it is also required to define the class of drug according to the biopharmaceutical classification system.ObjectivesThis study aimed to generate solubility data to obtain a complete pH Read More

PurposeA simple, rapid and precise reverse phase–High performance liquid chromatography (RP-HPLC) method was developed for the determination of degradation impurity i.e. Oxidised follitropin in recombinant Follicle Stimulating Hormone (rFSH) Injection.MethodsChromatographic separation was performed using a C4 column of size: 250 × 4.6 mm, 5 µm along with C3 Guard Colu Read More

ObjectiveA novel stability indicating RP-HPLC method was developed and validated for the simultaneous estimation of Pregabalin and Duloxetine by using the QBD approach.MethodsTo determine the optimal parameters for the duloxetine and pregabalin study, we used Design Expert version 13.0 in this work. The mobile phase consisted of 0.8 ml/min of methanol: water (80:20, v/v) with o-phosphoric acid-adjusted pH 3.A 250 mm Read More

Background and ObjectiveLipopolysaccharide (LPS) is an essential constituent of the outer membrane of gram-negative bacteria, such as Salmonella typhimurium, and it plays a crucial role by inducing disease in the host. Penicillin-binding protein 1B (LpoB) is a key enzyme in the production of peptidoglycans, making it a potential target for the development of new antimicrobials. Flavonoids are naturally occurring Read More

BackgroundOxaliplatin, a platinum-based antineoplastic agent, is widely used to treat colorectal cancer. It is well-known for its capacity to hinder the development and division of cells, especially fast-dividing ones, like cancer cells, via the creation of DNA adduct. The currently published oxaliplatin analytical methods require a complex, difficult-to-understand procedure, and are costly.ObjectiveThe main objectives of our s Read More

IntroductionA novel chemosensor has been developed utilizing a newly synthesized pyridine-carboxaldehyde thiosemicarbazone silver nanoprobe (PT-AgNP) for detecting and quantifying iodide ions in aqueous media, both visually and spectrophotometrically using a UV-Vis spectrophotometer.MethodsNotably, this sensor demonstrates remarkable selectivity for I- ions, effectively distinguishing them from oth Read More

IntroductionMangiferin shows great promise as a potent drug for a wide variety of diseases. Its low bioavailability and poor water solubility, however, restrict its therapeutic use.MethodsThe aim of the study goal was to systematically design a UV-spectroscopy method for mangiferin quantification in analytical samples that is quick, easy, and very sensitive. In order to validate the method, UV spectroscopy was used to Read More

BackgroundTriamterene is a potassium-conserving diuretic mainly used in the treatment of edematous diseases. Although there are some methods to measure the content of triamterene, the existing methods have the characteristics of sample destructiveness and low flux, making it difficult to meet the needs of online monitoring. In recent years, transmission Raman spectroscopy (TRS), as a new technology for the determinati Read More