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Preparation, Characterization and Quantification of Mangiferin in Mangiferin-loaded Microspheres
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- 28 May 2024
- 10 Sep 2024
Abstract
Introduction
Mangiferin shows great promise as a potent drug for a wide variety of diseases. Its low bioavailability and poor water solubility, however, restrict its therapeutic use.
Methods
The aim of the study goal was to systematically design a UV-spectroscopy method for mangiferin quantification in analytical samples that is quick, easy, and very sensitive. In order to validate the method, UV spectroscopy was used to check for specificity, accuracy, precision, and linearity. The models were constructed with Design ExpertⓇ V.10 and optimised using Box-Behnken Design (BBD), a three-factor and three-level procedure.
Results
The devised method demonstrated good levels of sensitivity, specificity, accuracy, and precision, according to the results. The absorbance and concentration showed a strong linear relationship in the given 5-25 μg/ml range for several wavelengths, with correlation coefficients of 0.989, 0.982, 0.905, and 0.896 at 364.5, 370, 378, and 265nm, respectively. The intraday precision was 0.788 at 364.5 nm, 0.801 at 370 nm, 0.739 at 378 nm, and 0.721 at 256 nm for the concentration of 20 μg/ml. Particle size and entrapment efficiency, two dependent variables in the microspheres formulation, were best suited by the models derived when the observed responses were fitted to the design. Microspheres had a high entrapment efficiency and were microsized. Interestingly, drug-encapsulated microspheres containing mangiferin maintained the spherical shape.
Conclusion
The existence of well-resolved peaks and good recovery of mangiferin in the analytical method for analysis makes it ideal and acceptable for further use.
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