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2000
Volume 20, Issue 8
  • ISSN: 1573-4129
  • E-ISSN: 1875-676X

Abstract

Background

(a Tonic-Qi traditional Chinese medicine) can enhance cellular metabolism and increase the lifespan of cells. Spermidine can markedly extend the lifespan. On the basis of these considerations, we assumed whether any relationship existed between the anti-aging activity of and spermidine.

Methods

In this research, spermidine and 10 other kinds of biogenic amines in samples were identified and determined using high performance liquid chromatography-diode array detector (HPLC-DAD) with precolumn derivatization. Biogenic amines were extracted with hydrochloric acid, purified by -butyl alcohol-dichloromethane extraction, and reacted with dansyl chloride for derivatization before HPLC analysis.

Results

The method had good repeatability and reproducibility for the quantitative determination of biogenic amines in samples. Putrescine, spermidine, and spermine were detected in 10 batches of samples. The total biogenic amines concentration was 48.09±2.22 mg/kg. Spermidine (22.84±2.59 mg/kg) was the highest amine in samples, followed by putrescine and spermine. This is the first report that contains biogenic amines, especially high content spermidine.

Conclusion

The high content of spermidine might be the one reason for with Tonic-Qi and anti-aging effect.

© 2024 Bentham Science Publishers
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Determination of Spermidine and other 10 Biogenic Amines in Radix astragali (Huang qi) by HPLC-DAD with Precolumn Derivatization
Current Pharmaceutical Analysis 20, 755 (2024); https://doi.org/10.2174/0115734129300505240813055500
/content/journals/cpa/10.2174/0115734129300505240813055500
/content/journals/cpa/10.2174/0115734129300505240813055500
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  • Article Type:     Research Article
Keyword(s): anti-aging activity; biogenic amines; HPLC-DAD; putrescine; Radix astragali; spermidine

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