Skip to content
2000
  1. Home
  2. A-Z Publications
  3. Current Pharmaceutical Analysis
  4. Volume 20, Issue 8
  5. Article
Volume 20, Issue 8
  • ISSN: 1573-4129
  • E-ISSN: 1875-676X

Abstract

Background

(a Tonic-Qi traditional Chinese medicine) can enhance cellular metabolism and increase the lifespan of cells. Spermidine can markedly extend the lifespan. On the basis of these considerations, we assumed whether any relationship existed between the anti-aging activity of and spermidine.

Methods

In this research, spermidine and 10 other kinds of biogenic amines in samples were identified and determined using high performance liquid chromatography-diode array detector (HPLC-DAD) with precolumn derivatization. Biogenic amines were extracted with hydrochloric acid, purified by -butyl alcohol-dichloromethane extraction, and reacted with dansyl chloride for derivatization before HPLC analysis.

Results

The method had good repeatability and reproducibility for the quantitative determination of biogenic amines in samples. Putrescine, spermidine, and spermine were detected in 10 batches of samples. The total biogenic amines concentration was 48.09±2.22 mg/kg. Spermidine (22.84±2.59 mg/kg) was the highest amine in samples, followed by putrescine and spermine. This is the first report that contains biogenic amines, especially high content spermidine.

Conclusion

The high content of spermidine might be the one reason for with Tonic-Qi and anti-aging effect.

© 2024 Bentham Science Publishers
Loading

Article metrics loading...

/content/journals/cpa/10.2174/0115734129300505240813055500
2024-08-28
2025-07-11

  • From This Site

    /content/journals/cpa/10.2174/0115734129300505240813055500
    dcterms_title,dcterms_subject,pub_keyword
    -contentType:Contributor -contentType:Concept -contentType:Institution
    10
    5
Loading full text...

Full text loading...

References

  1. SantosM.H.S. Biogenic amines: Their importance in foods.Int. J. Food Microbiol.1996292-321323110.1016/0168‑1605(95)00032‑18796424
     [Google Scholar]
  2. TassoniA. GermanàM. BagniN. Free and conjugated polyamine content in Citrus sinensis Osbeck, cultivar Brasiliano N.L. 92, a Navel orange, at different maturation stages.Food Chem.200487453754110.1016/j.foodchem.2004.01.001
     [Google Scholar]
  3. Kalac̆P. KrausováP. A review of dietary polyamines: Formation, implications for growth and health and occurrence in foods.Food Chem.2005901-221923010.1016/j.foodchem.2004.03.044
     [Google Scholar]
  4. EisenbergT. KnauerH. SchauerA. BüttnerS. RuckenstuhlC. Carmona-GutierrezD. RingJ. SchroederS. MagnesC. AntonacciL. FussiH. DeszczL. HartlR. SchramlE. CriolloA. MegalouE. WeiskopfD. LaunP. HeerenG. BreitenbachM. Grubeck-LoebensteinB. HerkerE. FahrenkrogB. FröhlichK.U. SinnerF. TavernarakisN. MinoisN. KroemerG. MadeoF. Induction of autophagy by spermidine promotes longevity.Nat. Cell Biol.200911111305131410.1038/ncb197519801973
     [Google Scholar]
  5. ChenG.H. HuangW.F. Progress in pharmacological effects of compositions of Astragalus membranaceus.Zhongguo Xin Yao Zazhi2008171482
     [Google Scholar]
  6. Deepika PriyadarshaniW.M. RakshitS.K. Screening selected strains of probiotic lactic acid bacteria for their ability to produce biogenic amines (histamine and tyramine).Int. J. Food Sci. Technol.201146102062206910.1111/j.1365‑2621.2011.02717.x
     [Google Scholar]
  7. Simon SarkadiL. Biogenic amines in fermented foods and health implications.Fermented Foods in Health and Disease PreventionAcademic Press20172762565110.1016/B978‑0‑12‑802309‑9.00027‑3
     [Google Scholar]
  8. MolognoniL. DaguerH. de Sá PloêncioL.A. De Dea LindnerJ. A multi-purpose tool for food inspection: Simultaneous determination of various classes of preservatives and biogenic amines in meat and fish products by LC-MS.Talanta20181781053106610.1016/j.talanta.2017.08.08129136796
     [Google Scholar]
  9. SuzziG. GardiniF. Biogenic amines in dry fermented sausages: A review.Int. J. Food Microbiol.2003881415410.1016/S0168‑1605(03)00080‑114527784
     [Google Scholar]
  10. Ruiz-CapillasC. Jiménez-ColmeneroF. Biogenic amines in meat and meat products.Crit. Rev. Food Sci. Nutr.2004447-848959910.1080/1040869049048934115969322
     [Google Scholar]
  11. ForsytheW.I. RedmondA. Two controlled trials of tyramine in children with migraine.Dev. Med. Child Neurol.197416679479910.1111/j.1469‑8749.1974.tb03400.x4613591
     [Google Scholar]
  12. BardóczS. Polyamines in food and their consequences for food quality and human health.Trends Food Sci. Technol.199561034134610.1016/S0924‑2244(00)89169‑4
     [Google Scholar]
  13. ÇiğdemS. HüseyinB. SongülS.E. Significance of biogenic amines in foods and their reduction methods.J. Food Eng.20138395410
     [Google Scholar]
  14. YuanH. ZhangS.F. JiaS.H. ChenN. Progress in application and biological activity of radix astragali in health foods.Shipin Kexue201435330
     [Google Scholar]
  15. WagnerH. BauerR. MelchartD. XiaoP.G. StaudingerA. Chromatographic Fingerprint Analysis of Herbal Medicines.ViennaSpringer-Verlag Wien201110.1007/978‑3‑7091‑0763‑8
     [Google Scholar]
  16. ChenK. LiC. Recent advances in studies on traditional Chinese anti-aging materia medica.J. Tradit. Chin. Med.19931332232268246603
     [Google Scholar]
  17. KvasničkaF. VoldřichM. Determination of biogenic amines by capillary zone electrophoresis with conductometric detection.J. Chromatogr. A20061103114514910.1016/j.chroma.2005.11.00516310200
     [Google Scholar]
  18. HeL. XuZ. HirokawaT. ShenL. Simultaneous determination of aliphatic, aromatic and heterocyclic biogenic amines without derivatization by capillary electrophoresis and application in beer analysis.J. Chromatogr. A2017148210911410.1016/j.chroma.2016.12.06728041619
     [Google Scholar]
  19. ÖnalA. TekkeliS.E.K. ÖnalC. A review of the liquid chromatographic methods for the determination of biogenic amines in foods.Food Chem.2013138150951510.1016/j.foodchem.2012.10.05623265518
     [Google Scholar]
  20. PinedaA. CarrascoJ. Peña-FarfalC. Henríquez-AedoK. ArandaM. Preliminary evaluation of biogenic amines content in Chilean young varietal wines by HPLC.Food Control201223125125710.1016/j.foodcont.2011.07.025
     [Google Scholar]
  21. CunhaS.C. FariaM.A. FernandesJ.O. Gas chromatography-mass spectrometry assessment of amines in Port wine and grape juice after fast chloroformate extraction/derivatization.J. Agric. Food Chem.201159168742875310.1021/jf201379x21732693
     [Google Scholar]
  22. PalermoC. MuscarellaM. NardielloD. IammarinoM. CentonzeD. A multiresidual method based on ion-exchange chromatography with conductivity detection for the determination of biogenic amines in food and beverages.Anal. Bioanal. Chem.20134052-31015102310.1007/s00216‑012‑6439‑z23052881
     [Google Scholar]
  23. Lapa-GuimarãesJ. PickovaJ. New solvent systems for thin-layer chromatographic determination of nine biogenic amines in fish and squid.J. Chromatogr. A200410451-222323210.1016/j.chroma.2004.06.01415378899
     [Google Scholar]
  24. RomanoA. KlebanowskiH. La GuercheS. BeneduceL. SpanoG. MuratM.L. LucasP. Determination of biogenic amines in wine by thin-layer chromatography/densitometry.Food Chem.201213531392139610.1016/j.foodchem.2012.06.02222953871
     [Google Scholar]
  25. De MeyE. Drabik-MarkiewiczG. De MaereH. PeetersM.C. DerdelinckxG. PaelinckH. KowalskaT. Dabsyl derivatisation as an alternative for dansylation in the detection of biogenic amines in fermented meat products by reversed phase high performance liquid chromatography.Food Chem.201213041017102310.1016/j.foodchem.2011.07.124
     [Google Scholar]
  26. CostaM.P. BalthazarC.F. RodriguesB.L. LázaroC.A. SilvaA.C.O. CruzA.G. Conte JuniorC.A. Determination of biogenic amines by high-performance liquid chromatography (HPLC-DAD) in probiotic cow’s and goat’s fermented milks and acceptance.Food Sci. Nutr.20153317217810.1002/fsn3.20025987991
     [Google Scholar]
  27. YangY.X. MuC.L. ZhangJ.F. ZhuW.Y. Determination of biogenic amines in digesta by high performance liquid chromatography with precolumn dansylation.Anal. Lett.20144781290129810.1080/00032719.2013.871550
     [Google Scholar]
  28. ÖnalA. A review: Current analytical methods for the determination of biogenic amines in foods.Food Chem.200710341475148610.1016/j.foodchem.2006.08.028
     [Google Scholar]
  29. AiY. SunY.N. LiuL. YaoF.Y. ZhangY. GuoF.Y. ZhaoW.J. LiuJ.L. ZhangN. Determination of biogenic amines in different parts of Lycium barbarum L. by HPLC with precolumn dansylation.Molecules2021264104610.3390/molecules2604104633671270
     [Google Scholar]
  30. SunY. AiY. YaoF. MaoF. WangX. ZhangY. GuoF. HeY. LiuJ. ZhangN. Characterization of biogenic amines in seven kinds of traditional chinese animal medicines by high performance liquid chromatography with precolumn derivatization.J. Anal. Chem.2022771818610.1134/S1061934822010166
     [Google Scholar]
  31. JiaS. KangY.P. ParkJ.H. LeeJ. KwonS.W. Simultaneous determination of 23 amino acids and 7 biogenic amines in fermented food samples by liquid chromatography/quadrupole time-of-flight mass spectrometry.J. Chromatogr. A20111218519174918210.1016/j.chroma.2011.10.04022074649
     [Google Scholar]
  32. YangS.S. YangY.N. LiX-L. ZhangY. Determination of biogenic amines in cheese by on-line solid phase extraction coupled with capillary high performance liquid chromatography.Chin. J. Anal. Chem.201644339640210.1016/S1872‑2040(16)60915‑5
     [Google Scholar]
  33. Paulo VieiraC. Pereira da CostaM. SilvaV.L.M. da Silva FrasaoB. Campos de AquinoL.F.M. de Oliveira NunesY.E.C. Adam Conte-JuniorC. Development and validation of RP-HPLC-DAD method for biogenic amines determination in probiotic yogurts.Arab. J. Chem.20201311582159710.1016/j.arabjc.2017.12.010
     [Google Scholar]
  34. ZhangN. WangY.Q. WangX.N. FanM.Q. SunY.N. LiuZ.L. LiuJ.L. Identification and determination of biogenic amines in nine kinds of Chinese Tonic-Qi herbs by RP-HPLC with pre- column derivatization.Yaowu Fenxi Zazhi2017371797
     [Google Scholar]
  35. ZhangN. Derivatization analysis of biogenic amines and amino acid in traditional Chinese Medicine.Xi’anNorthwest University2014
     [Google Scholar]
  36. SunX.S. YangL. Research progress of anti-aging mechanisms and drugs.J. Guangzhou Univ. Tradit. Chin. Med.200926593597
     [Google Scholar]
  37. WuJ.S. LiJ.M. LoH.Y. HsiangC.Y. HoT.Y. Anti-hypertensive and angiotensin-converting enzyme inhibitory effects of Radix Astragali and its bioactive peptide AM-1.J. Ethnopharmacol.202025411272410.1016/j.jep.2020.11272432119952
     [Google Scholar]
/content/journals/cpa/10.2174/0115734129300505240813055500
Loading
Determination of Spermidine and other 10 Biogenic Amines in Radix astragali (Huang qi) by HPLC-DAD with Precolumn Derivatization
Current Pharmaceutical Analysis 20, 755 (2024); https://doi.org/10.2174/0115734129300505240813055500
/content/journals/cpa/10.2174/0115734129300505240813055500
/content/journals/cpa/10.2174/0115734129300505240813055500
Loading

Data & Media loading...

Supplements

Supplementary material is available on the publisher’s website along with the published article.

file format download Download

  • Article Type:     Research Article
Keyword(s): anti-aging activity; biogenic amines; HPLC-DAD; putrescine; Radix astragali; spermidine

Most Cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error
Please enter a valid_number test