Skip to content
2000
Volume 20, Issue 8
  • ISSN: 1573-4129
  • E-ISSN: 1875-676X

Abstract

Background

Hispidulin, a flavonoid renowned for its diverse pharmacological activities, is abundant in D. Don. Given its prevalence, it is essential to establish a dependable analytical method for quantifying hispidulin in this plant.

Methods

This study introduces a sensitive and reliable high-performance thin-layer chromatographic (HPTLC) method for both qualitative and quantitative analysis of hispidulin in D. Don. Pre-coated TLC aluminum plates were utilized, and after testing various mobile phase combinations, the most effective separation was achieved with Toluene-ethyl acetate-formic acid (5:4:1, v/v/v). The method underwent extensive validation for linearity, specification, instrument precision, precision, limits of LOD and LOQ, accuracy, and robustness.

Results

The developed method demonstrated excellent linearity over a specified concentration range, with a determination coefficient (r2) of 0.988. A well-defined spot at Rf 0.7, corresponding to hispidulin, was observed. The concentration of hispidulin in the hydroethanolic extract of D. Don was quantified as 13.02 μg/mg of extract.

Conclusion

In conclusion, the HPTLC method presented in this study offers a simple, accurate, and robust tool for quantitatively analyzing hispidulin in the hydroethanol extract of D. Don. This method is suitable for routine quality control and standardization of herbal formulations containing D. Don, thereby contributing to the advancement of research in natural product-based pharmaceuticals.

Loading

Article metrics loading...

/content/journals/cpa/10.2174/0115734129307814240906065932
2024-09-27
2025-07-08
Loading full text...

Full text loading...

References

  1. ChenQ. RahmanK. WangS.J. ZhouS. ZhangH. Scutellaria barbata: A review on chemical constituents, pharmacological activities and clinical applications.Curr. Pharm. Des.202026116017510.2174/138161282566619121612431031840605
    [Google Scholar]
  2. WangL. ChenW. LiM. ZhangF. ChenK. ChenW. A review of the ethnopharmacology, phytochemistry, pharmacology, and quality control of Scutellaria barbata D. Don.J. Ethnopharmacol.202025411226010.1016/j.jep.2019.11226031577937
    [Google Scholar]
  3. ChenY. SunJ. ZhangZ. LiuX. WangQ. YuY. The potential effects and mechanisms of hispidulin in the treatment of diabetic retinopathy based on network pharmacology.BMC Complement Med Ther.202222114110.1186/s12906‑022‑03593‑235590353
    [Google Scholar]
  4. KavvadiasD. SandP. YoudimK.A. QaiserM.Z. Rice-EvansC. BaurR. SigelE. RauschW.D. RiedererP. SchreierP. The flavone hispidulin, a benzodiazepine receptor ligand with positive allosteric properties, traverses the blood–brain barrier and exhibits anticonvulsive effects.Br. J. Pharmacol.2004142581182010.1038/sj.bjp.070582815231642
    [Google Scholar]
  5. AttimaradM. Mueen AhmedK.K. AldhubaibB.E. HarshaS. High-performance thin layer chromatography: A powerful analytical technique in pharmaceutical drug discovery.Pharm. Methods201122717510.4103/2229‑4708.8443623781433
    [Google Scholar]
  6. EvansW.C. Trease and Evans Pharmacognosy.15th edPhiladelphiaW.B. Saunders Company Ltd.2002
    [Google Scholar]
  7. GökbulutA. Determınatıon of hıspıdulın in the flowers of inula vıscosa (L.) aıton usıng HPLC and HPTLC methods.Turk J Pharm Sci.2016132334010.5505/tjps.2016.47955
    [Google Scholar]
  8. HeydenY.V. Extracting information from chromatographic herbal fingerprints.LC GC Eur.200821438443
    [Google Scholar]
  9. LoescherC.M. MortonD.W. RazicS. Agatonovic-KustrinS. High performance thin layer chromatography (HPTLC) and high performance liquid chromatography (HPLC) for the qualitative and quantitative analysis of Calendula officinalis—Advantages and limitations.J. Pharm. Biomed. Anal.201498525910.1016/j.jpba.2014.04.02324880991
    [Google Scholar]
  10. AmirM. MujeebM. AhmadS. AkhtarM. AshrafK. Design expert-supported development and validation of HPTLC method: An application in simultaneous estimation of quercetin and rutin in Punica granatum, Tamarindus indica and Prunus domestica.Pharm. Methods201342626710.1016/j.phme.2013.12.004
    [Google Scholar]
  11. AkowuahG.A. ZhariI. SadikunA. NorhayatiI. Leaf Extracts and Inhibitory Effect on Xanthine Oxidase Activity HPTLC densitometric analysis of Orthosiphon stamineus leaf extracts and inhibitory effect on xanthine oxidase activity.Pharm. Biol.2006441657010.1080/13880200500531045
    [Google Scholar]
  12. BholeR.P. ShindeS.S. ChitlangeS.S. WankhedeS.B. A high-performance thin layer chromatography (HPTLC) method for simultaneous determination of diphenhydramine hydrochloride and naproxen sodium in tablets.Anal. Chem. Insights201510ACI.S3150610.4137/ACI.S3150626692760
    [Google Scholar]
  13. SyamasundarB. SuneethaA. Development and validation of HPTLC method for the estimation of almotriptan malate in tablet dosage form.Indian J. Pharm. Sci.201072562963210.4103/0250‑474X.7853421694997
    [Google Scholar]
  14. KambojA. SalujaA.K. Development of validated HPTLC method for quantification of stigmasterol from leaf and stem of Bryophyllum pinnatum.Arab. J. Chem.2013715210.1016/j.arabjc.2013.10.006
    [Google Scholar]
  15. MathurR. Jayachandran NairC.V. AhamadS. KhanW. AnjumV. Development and validation of high-performance thin-layer chromatography method for simultaneous determination of polyphenolic compounds in medicinal plants.Pharmacognosy Res.201795Suppl. 16710.4103/pr.pr_122_1629333046
    [Google Scholar]
  16. GB. SuripeddiR.K. Development and validation of HPTLC method for identification and quantification of sterols from leaves of Erythroxylum monogynum Roxb. and in vitro evaluation of anti-oxidant and anti-glycation activities.S. Afr. J. Bot.2021137243410.1016/j.sajb.2020.10.005
    [Google Scholar]
  17. MajidA.M.S.A. HashimS. BehH.K. HamilM.S.R. IsmailZ. High-performance thin-layer chromatography method development, validation, and simultaneous quantification of four compounds identified in standardized extracts of Orthosiphon stamineus. Pharmacognosy Res.20168423824310.4103/0974‑8490.18887227695261
    [Google Scholar]
  18. SreekanthN. AwenB. RaoC.B. HPTLC method development and validation of trandolapril in bulk and pharmaceutical dosage forms.J. Adv. Pharm. Technol. Res.20101217217910.4103/2231‑4040.7225522247843
    [Google Scholar]
  19. HorwitzW. AOAC guidelines for single laboratory validation of chemical methods for dietary supplements and botanicals.Gaithersburg, MD, USAAOAC International20021219
    [Google Scholar]
/content/journals/cpa/10.2174/0115734129307814240906065932
Loading
/content/journals/cpa/10.2174/0115734129307814240906065932
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error
Please enter a valid_number test