Anti-Infective Agents - Online First

The urgent need for new antimicrobial compounds arises from the growing threat of multidrug-resistant human pathogens responsible for infectious diseases. The indole moiety, a prevalent heterocyclic ring system found in nature, is a key structural element in many pharmaceutical agents due to its wide range of biological activities. Bis(indolyl)methanes, in particular, have emerged as promising candidates for antibacterial activity.

This study aimed to evaluate the antibacterial activity of nine bis(indolyl)methane derivatives against a range of pathogenic bacterial strains responsible for various human diseases.

The compounds were synthesized using a solvent-free method, and their antibacterial activity was evaluated using the disk diffusion assay. The minimum inhibitory concentration (MIC) of the active compounds identified in the disk diffusion assay was determined by the microtiter broth dilution method in 96-well microtiter plates. Bacterial strains in the mid-log phase of growth were utilized. Bacterial suspensions equivalent to 0.5 McFarland standards were prepared by suspending the bacterial inoculum in sterile water. A working concentration of 100 µg/mL was achieved by diluting the test compounds in 100% DMSO.

The antimicrobial activity of nine synthetic compounds was evaluated against nine medically significant pathogenic strains. These include Pseudomonas aeruginosa, Bacillus cereus, and Shigella flexneri, known for producing toxins that cause acute foodborne illnesses, as well as Escherichia coli K12 and Enterococcus faecalis, which can disrupt the intestinal barrier in immunocompromised individuals. The results suggest that these compounds have the potential to be effective antimicrobial agents.

Our findings demonstrate the promising antimicrobial activity of the synthesized compounds, with 1-ethyl-3-((1-ethyl-1H-indol-3-yl)(phenyl)methyl)-1H-indole emerging as the most potent, significantly inhibiting most tested bacterial strains. These results highlight the potential for developing novel compounds for antibacterial treatment.

Cancer, diabetes, and infection of bacteria and fungi are serious issues in the world. Synthesis of the potential drugs against them is a major challenge to the researcher. Hence, coordination chemistry has attracted researchers as it has various applications in medicinal and biological fields.

Synthesis and biological evolution of azo-Schiff base ligand and its Mn(II), Co(II), Ni(II), Cu(II), Zn(II), and VO(II) metal complexes were carried out. These compounds were characterized by Mass, 1H-NMR, FT-IR, Elemental analysis, Molar conductance, Magnetic susceptibility, UV-Vis., P-XRD, TGA, etc., and were screened for biological activities.

Synthesised azo-Schiff base ligand and its metal complexes were evaluated for their antimicrobial, antidiabetic as well as anticáncer activities against various bacteria and fungi and MCF-7 breast cancer cell line, respectively.

From the findings of various results, we can conclude that the synthesized metal complexes exhibit higher biological activities than the azo-Schiff base ligand.

Microbes are a rich source of antibacterial and anti-insect molecules. Due to rising antibiotic and anti-insect resistance in various sectors of the society, it is important to identify new compounds that may address these issues.

This study aimed to explore the bacteria isolated from soil to identify new molecules with antibacterial and anti-insect activity. Further, the current study is aimed at testing and characterizing antimicrobial and insecticidal properties of methanolic extracts from four different soil bacteria

This study reports the isolation and characterization of soil bacteria by morphological, biochemical, and molecular analysis. The antibacterial potential of methanolic extracts of four bacterial strains were tested using an agar well diffusion assay, along with development and survival of Spodoptera litura. Fractionation of the methanolic extract was performed by chromatography, and the separated fractions were tested for their antibacterial activity.

The bacteria belong to Bacillus, Exiguobacterium, and Microbacterium species. The extract of Bacillus licheniformis SKS7 exhibited maximum antibacterial activity against all tested microbes, including human pathogens. Extract from the same microbe also showed maximum anti-insect activity against Spodoptera litura by significantly increasing the pupal period by as much as 80% and hence extending the time to adult emergence. Morphological abnormalities like deformed wings, deformed pupae, and failure to emerge from pupae were also observed. Purification of the extract by HPLC and gel permeation chromatography helped us to observe a low molecular weight protein that may be responsible for its antibacterial activity.

Methanolic extract of Bacillus licheniformis SKS7 contains bioactive molecules with antibacterial and anti-insect activities. Further characterization and identification of these molecules may form the basis for the development of novel antibacterial drugs and insecticidal molecules in the future.

from microbes has slowed since the late 1980s due to hardships in finding novel active compounds and reg