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2000
Volume 4, Issue 2
  • ISSN: 2210-3031
  • E-ISSN: 2210-304X

Abstract

Synthetic DNA molecules of 10-23 deoxyribozymes (Dz) are known to be efficient and site-specific agents for the RNA cleavage in a catalytic manner. To provide the penetration of Dz into cells, we have proposed the new delivery system for deoxyribozymes in the form of TiO2•PL-Dz nanocomposite. These nanocomposites consist of catalytically active Dz molecules noncovalently immobilized through the polylysine linker to TiO2 nanoparticles, which provide the penetration of Dz into cells. Deoxyribozymes in the prepared TiO2•PL-Dz nanocomposites were shown to retain their ability to cleave RNA targets albeit at a slower rate but with the same site-specificity and similar efficiency as free Dz. Unlike free Dz, the proposed TiO2•PL-Dz nanocomposites penetrate into cells without auxiliary actions.

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/content/journals/ddl/10.2174/2210303104666140222002510
2014-08-01
2025-06-11
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/content/journals/ddl/10.2174/2210303104666140222002510
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  • Article Type:
    Research Article
Keyword(s): Delivery; deoxyribozymes; immobilization; nanocomposites; TiO2 nanoparticles
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