Current Pharmaceutical Analysis - Volume 17, Issue 8, 2021

Pharmaceutical analysis plays an important role in all steps of drug development processes. Analysis of active pharmaceutical ingredients in biological samples needs sample preparation techniques to prevent the signal of the analyte from interferences coming from matrix components. Ultrafiltration is a well-known technique used in the food and pharmaceutical industry. Commercial ultrafiltration devices have been freq Read More

Background: Metallic impurities are the traces of metals that can be found in finished drug products. Description: These metallic impurities in pharmaceutical preparations can enter from formulation ingredients, catalysts, and process equipment, containers and closures. They are not completely removed from the product by practical manufacturing techniques and should be evaluated relative to safetybased limits. Th Read More

Every "entity" or compound has physical and chemical properties as references for the synthesis and determination of the entity's structure. Thermodynamically, solid-state is the most stable matter in the universe and to be the ideal form in structure elucidation of pharmaceutical. The dry treatments, such as mechanochemistry, microwave heating, and the using of deep eutectic agent are becoming popular. These tec Read More

Background: Rifampicin (RIF), also known as rifampin, a bactericidal antibiotic having broad antibacterial activity against various gram-positive and gram-negative bacteria acts by inhibiting DNA dependent RNA polymerase. RIF has been administered in different dosage forms like tablets, capsules, injections, oral suspension, powder, etc. for the treatment of several types of bacterial infections, including tuberculosis, Myco Read More

Background: Udenafil, a recently discovered drug used for erectile dysfunction treatment, has been widely prescribed and its effect on human systems has been extensively studied. However, there is little research on the human metabolites of udenafil. Three metabolites have been identified in rats. Objective: Herein, highly sensitive and accurate liquid chromatography–quadrupole time-of-flight tandem mass spectro Read More

Background: The serum levels of Docosahexaenoic Acid (DHA), Eicosapentaenoic Acid (EPA) and Arachidonic Acid (AA) under the state of Pressure Ulcers (PUs) are still unclear. Introduction: In order to investigate serum levels of DHA, EPA, and AA in PUs rats, an ultraperformance liquid chromatography-tandem mass spectrometry (UPLC-MS/ MS) method was developed and validated. Methods: Chromatographic separati Read More

Background: Cuscutae Semen (CS) is reported to show a hepatoprotective effect. Chlorogenic acid, hyperoside and astragalin are three major biologically active components from CS. Objective: A sensitive method based on ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) was developed and validated to quantify the three components in rat plasma and was successfully used to Read More

Background: D-amino acids are closely related to the development and progression of Alzheimer's disease (AD) and are expected as the novel biomarkers for AD diagnosis. Objective: The aim was to investigate the potential clinical value of D-amino acids for Alzheimer's disease. Methods: A simple and sensitive HPLC/MS-MS method was developed for the simultaneous determination of D-alanine, D-glutamine, D-proline and D Read More

Introduction: This study introduces an effective strategy, which combines high performance liquid chromatography coupled with diode array detection (HPLC-DAD) with multivariate calibration methods for the simultaneous determination of paracetamol (PAR), pseudoephedrine HCl (PSE), dextromethorphan HBr (DEX) and doxylamine succinate (DOX) along with sweetener saccharin (SAC) in syrup formulation. Methods: PLS Read More

Background: N’,N’-diethyl-m-toluamide (DEET) is the most widely used repellent substance worldwide. It is formulated as aerosol, solution, lotion, gel and patches. However, the official compendia report monographs to analyze only DEET drug substance and solution. Objective: In this study, an isocratic HPLC method was validated to assay DEET in lotion, gel and solution, under the same analytical conditions. Methods: The meth Read More

Background: The development of sound bioanalytical LC-MS (liquid chromatography-mass spectroscopy) method(s) is of paramount importance during the process of drug discovery, development and culminating in a marketing approval. The use of oral antidiabetic agents has been increased significantly from the last decades and till now no bioanalytical method is available for quantitation of sitagliptin (SG) and ertugliflozin (E Read More

Background: An enantiomeric separation of the stability-indicating high-performance liquid chromatographic method was developed and validated for the analysis of Meclizine enantiomers. The degradation behavior of Meclizine Hydrochloride was investigated under different stress conditions recommended by the International Conference on Harmonization (ICH). Methods: Enantiomeric resolution of the drug and complete sep Read More

Background: In case of meningitis, the meninges are inflamed and the blood brain barrier is distorted, therefore, there is no hindrance to drug penetration. The problem arises when the disease is at the verge of cure, the meninges become uninflamed and the permeability of the drug is reduced to such extend that it becomes nearly impossible to maintain the minimum inhibitory concentration of drug at the site of infecti Read More

Background: Alfuzosin is recently co-formulated with solifenacin for relieving two coincident urological diseases, namely; benign prostate hyperplasia and overactive bladder. Objective: Herein, green, simple and rapid spectrophotometric methods were firstly developed for simultaneous determination of the two cited drugs in their co-formulated pharmaceutical capsule. Methods: Alfuzosin, which is the majo Read More

Background: PARTIAL Least Squares (PLS) and Principal Component Regression (PCR) are two well-known chemometric methods based on dimension reduction techniques. They can be very practical analyzing a large data set of multiple correlated predictor variables. Objective: In the presented work, the resolving power of spectrophotometric assisted mathematical techniques was implemented for the simultaneous d Read More