Current Pharmaceutical Analysis - Volume 17, Issue 9, 2021

Roxithromycin is one of the most frequently used macrolide antibiotics, a safe group of antimicrobials, that acts against Gram-positive bacteria and some Gram-negative bacteria. It is sold in several countries in different dosage forms (tablets and capsules) and strengths (50, 100, 150 and 300 mg). Several analytical methods have been described to quantify roxithromycin in different matrices, such as biological and environmen Read More

Background: Neuropathy is the most common perplexity of diabetes type 1 and 2. About 50% of diabetic patients develop diabetic neuropathic pain (DNP). At the beginning of diabetic neuropathy, there is a loss of sense, especially in the lower limb, accompanied by pain, and difficulty in movement. Glucose regulation effectively prevents the development of diabetic neuropathy in type 1 diabetic patients, but the consequ Read More

Pharmaceuticals are the chemical molecules which were invented to improve the life of living things through diagnosing, curing, treating, or preventing diseases. Pharmaceuticals can do their maximum function or work against disease or prevent from harmful effects of diseases only if they are free from impurities. The pharmaceutical analysis consists of different essential and important procedures to ascertain “identit Read More

Background: Since propofol is rapidly metabolized and excreted from the body, it is not easy to quantify its intake in blood or urine samples over the time. In this case, the hair sample would be more advantageous to estimate during the abuse period. However, the presence of protein and lipid in the hair sample could interfere with extraction and be problematic during mass spectrometric analysis. Objective: The aim of this Read More

Background: Morin flavonoid exerts neuroprotective effects with potential interest in neurodegenerative disorders. For this, the use of surface-modified polymeric nanoparticles loaded with morin is an interesting approach. Objective: To develop and validate an HPLC method for the quantification of morin released from a new delivery system consisting of poly lactic-co-glycolic (PLGA) nanoparticles functionalized with Read More

Background: The activation or overexpression of histone deacetylases (HDACs) are associated with the development of numerous cancers. The importance of HDACs in new anticancer therapeutic strategies has led researchers to develop promising anticancer drugs, design, and synthesis of novel HDACs inhibitors are also in progress. E14 was synthesized and acted as a selective inhibitor of HDACs I. Methods: We develop a s Read More

Background: Current methods for assaying bioactivity of GLP-1 analogues are costly and time consuming, mainly dependent on AlphaScreen luminous beads, based on fluorescent resonance energy transfer technology, and cAMP-GloTM Max assay kit, and on ATP to generate a negative- association curve. Objective: To establish a sensitive, stable, and low-cost method for the determination of biological activity of therapeu Read More

Objectives: Riptolide (TPL) has been shown to have a good clinical effect on rheumatoid arthritis (RA). We designed TPL microspheres (TPL-MS) and investigated its metabolic behavior in human, dog, rabbit and rat liver microsomes (HLM, DLM, RLM and SDRLM) with UPLC- MS/MS method. Methods: First, a UPLC-MS/MS method was established to measure the concentration of TPL in samples. The sample was separated on a Read More

Background: Cannabidiol (CBD) is the main, physiologically active, but psycho-inactive constituent of the glandular hairs of Cannabis sativa. CBD exhibits diverse pharmacological activities and it is used as an anticonvulsant, sedative and hypnotic agent. Other biological properties shown by CBD are antipsychotic, anti-inflammatory and neuroprotective. Objective: The aim of this study is to develop, validate and apply high per Read More

Aim: EPAS (evaporative precipitation into aqueous solution) was used in the current studies to prepare azithromycin nanosuspensions and investigate the physicochemical characteristics for the nanosuspension batches with the aim of enhancing the dissolution rate of the nanopreparation to improve bioavailability. Methods: EPAS method used in this study for preparing azithromycin nanosuspension was ac Read More

Background: The presence of residual solvents in pharmaceuticals can be a potential risk factor to human health because of its toxicity. Gas chromatography is used for its excellent separation abilities and lower limit of detection. Racecadotril, an antidiarrheal drug, is reported having four residual solvents, namely n-Hexane, isopropyl alcohol, toluene, and dimethylformamide. Estimation of the amount of these solvents in a Read More

Background: Haemodialysis is a process to remove the waste products and excess fluids that build up in the human body when kidneys stop working. Haemodialysis concentrate consists of two parts, acid concentrate (Part A) that contains mineral ions (Na, K, Mg and Ca) and buffering agent acetic acid /citric acid whereas basic concentrate (Part B) comprises sodium bicarbonate alone or in combination with sodium chl Read More