Current Pharmaceutical Analysis - Volume 17, Issue 4, 2021

Background: Quality by design-based software’s in analytical research and development normally encompasses multiple objectives. For decades, this task has been attempted through trial and error, supplemented with the previous experience, knowledge, and wisdom of analytical researchers. Objective: The study analyzes the current QbD-assisted software’s, such as design-experts, minitab, fusion product develop Read More

Background: Metabolomics is one of the main areas to understand cellular process at molecular level by analyzing metabolites. In recent years metabolomics has emerged as a key tool to understand molecular basis of diseases, to find diagnostic and prognostic biomarkers and develop new treatment opportunities and drug molecules. Objective: In this study, untargeted metabolite and lipid analysis were performed to identif Read More

Background: Drugs based on natural products targeting the microtubule system remain an important component in cancer therapy. Compound 10, 4-((3-amino-4-methoxyphenyl) amino)-2Hcoumarin, derived from coumarin, showed excellent anti-proliferative activity through directly binding to the colchicine-binding site in β-tubulin, suggesting that it could be a perfect drug candidate for antitumor drug research and developme Read More

Objective: The objective of the method was to develop a new, simple and reliable enantioselective Reverse Phase- Ultra-Fast Liquid Chromatography (RP-UFLC) method for the separation of Atenolol enantiomers. A comprehensive study was performed by extending the work to pharmacokinetic studies using rabbit plasma. Background: Many methods were reported for enantioseparation of Atenolol enantiomers Read More

Background: Pyrogens are fever-inducing substances and pyrogen detection is mandatory in parenteral pharmaceuticals. Succinylated Gelatin Injection (SGI) is a biopharmaceutical product, containing multi-component, and it is administered parenterally. Objective: The study aimed to assess pyrogen in SGI and to evaluate the feasibility of the Monocyte Activation Test (MAT) for pyrogen detection in a multi-component p Read More

Background: Although chromatography and spectrometry-based methods have been used to analyse phenolic acids in Chinese traditional medicine Salvia miltiorrhiza Bunge (SMB), quantitative nuclear magnetic resonance (qNMR) has never previously been used to analyse fresh SMB root extracts. Objective: To establish a fast and simple method of quantitating danshensu, lithospermic acid, rosmarinic acid, and salvianolic Read More

Introduction: Dryopteris marginalis L. is a local plant in Bangladesh which is widely used as folk medicine. Materials and Methods: In this study, the chemical composition of essential oil of D. marginalis L. as well as the antioxidant activity, antibacterial activity and total phenolic content of various organic extracts were analyzed. The gas chromatography mass spectrometry analysis was used to identify the chemical compositions. T Read More

Background: The indole-containing aroylhydrazone derivatives 3a-c with potent antimycobacterial activity against a referent strain M. tuberculosis H37Rv and low cytotoxicity were evaluated for their stability via the precise and accurate HPLC analytical method in aqueous media of different pH (2.0, 7.0, 9.0 and 12.0). Objective: The study describes the development and validation of a simple and reliable HPLC-UV procedure for t Read More

Background: Curcumin (CUR), a natural isolated compound from turmeric, helps in fighting many diseases, but the broad application of curcumin has been limited ascribed to low bioavailability. Objective: The aim of this study is to pursue the enhancement of curcumin bioavailability through coadministration of vitamin C. Methods: Such purpose was achieved through the analysis of curcumin pharmacokinetics by highperform Read More

Introduction: In this study, a precise, rapid and accurate ultra-performance liquid chromatography- tandem mass spectrometry (UPLC-MS/MS) method for the quantification of zhebeirine in mouse blood was developed, and pharmacokinetics of zhebeirine was studied for the first time after intravenous and oral administration. Methods: The mobile phase consisted of acetonitrile-0.1% formic acid, with a flow rate at 0.4 mL/m Read More

Background: A simple quantification technique by liquid chromatography electrospray ionization- tandem mass spectrometry (LC-ESI-MS/MS) is required for regorafenib in biological matrices with bioavailability studies in healthy rabbits, when compared with reported techniques. Objective: The main aim of the research work was to develop a validated LC-ESI-MS/MS technique for the quantification of regorafenib and applica Read More

Introduction: Impurity analysis is an important step in the quality control of pharmaceutical ingredients and the final product. Impurities can arise from drug synthesis or excipients and even at small concentrations may affect product efficacy and safety. In this work, two methods using highperformance liquid chromatography (HPLC) were developed and validated for the evaluation of besifloxacin and its impurity synthes Read More