Current Pharmaceutical Analysis - Volume 17, Issue 5, 2021

Introduction: Epilepsy is one of the most common neurological diseases in clinical practice. The combined application of metabolomics technology plays a great advantage in the screening of biomarkers. Methods: In this study, Wistar rats were used as experimental subjects to model intractable epilepsy and to detect the metabolic changes of small molecules in plasma. UPLC-MS/MS was used to determine the small molecules in Read More

Background: Up to date, generic linezolid injections produced by Chinese manufacturers were not widely used in clinics in China. Quality evaluation of linezolid injections produced in China is a prerequisite, which has rarely been performed. Objective: This study aimed to evaluate the quality of branded and generic injections from different manufacturers and to provide a basis for quality control. Methods: In this study, the conte Read More

Objective: Apatinib, a novel small-molecule Tyrosine Kinase Inhibitor (TKI), is under development to treat advanced gastric cancer. For the pharmacokinetic evaluation and routine drug monitoring of apatinib, a quantitative ultra-performance liquid chromatography coupled with tandem mass spectrometry (UPLC-MS/MS) method in rat plasma was developed with tinidazole used as an internal standard (IS). Methods: Protei Read More

Introduction: Spectrophotometry was investigated for the determination of epsilon aminocaproic acid (EACA) with p-nitrophenol (PNP). The method was based on Charge Transfer (CT) complexation of this drug as n-electron donor with π-acceptor PNP. Methods: The experiment indicated that CT complexation was carried out at room temperature for 10 minutes in dimethyl sulfoxide solvent. The spectrum obtained for EACA/PNP s Read More

Background: Polyethylene glycol 400 (PEG400), as a good traditional Chinese medicine solvent, diluent and solubilizer, is widely used as a main pharmaceutical excipient in traditional Chinese medicine compound preparations containing active ingredient baicalin. PEG400 could increase the solubility and release of baicalin in vivo, but it was unknown that PEG400 affected the absorption and distribution of baicalin or not. Obje Read More

Background: Levocetirizine is chemically know as (R)-(-)-2-[4-(4-chlorobenzhydryl)-1- piperazinyl]-ethoxy acetic acid dihydrochloride. Many publications have reported the synthetic routes of levocetirizine. Several related substances have been detected in levocetirizine hydrochloride drug substances. In our study, a pharmaceutical impurity, at the level of >0.1% w/w, was isolated, purified ed and identified. It is not incl Read More

Background: Danyikangtai powder, a Traditional Chinese Medicine (TCM) formula, shows promise to become a novel drug candidate for the simultaneous treatment of chronic cholecystitis and chronic pancreatitis. However, the pharmacokinetic behavior of Danyikangtai powder remains unclear. Objective: We investigated the comparative pharmacokinetics of four flavonoids in rats after oral administration of Danyikangta Read More

Aims: Characterization of antimicrobial metabolites of novel Streptomyces sp. UK-238. Background: Novel antimicrobial drug discovery is urgently needed due to emerging multi antimicrobial drug resistance among pathogens. Since many years, natural products have provided the basic skeletons for many therapeutic compounds including antibiotics. Bioprospection of un/under explored habitats and focussing on selectiv Read More

Background: The determination of trace drugs in aquatic environments is important. For this purpose, many methods such as High Performance Liquid Chromatography (HPLC) and gas chromatography mass spectrometry (GC/MS) have been used. Objective: This study introduces a simple, sensitive, and rapid colorimetric method for the spectrophotometric determination of sitagliptin (STG) in drinking water, tablet, human p Read More

Background: Haloperidol (HP) and Risperidone (RIS) are antipsychotic drugs and the simultaneous determination of these drugs is important. Estimation of HP and RIS alone or in combination with other drugs has been performed in a variety of ways. Objective: The aim of this paper was to propose a rapid, simple, accurate, and robust method for the simultaneous determination of HP and RIS using Artificial Neural Networks Read More

Background: Sample preparation is one of the most challenging phases in pharmaceutical analysis, especially in biological matrices, affecting the whole analytical methodology. Objective: In this study, a new Ca(II)/Cu(II)/alginate/CuO Nanoparticles Hydrogel Fiber (CCACHF) was synthesized through a simple, green procedure and applied for fiber micro solid-phase extraction (FMSPE) of diazepam (DIZ) and oxazepam (OXZ) a Read More

Introduction: A reverse-phase high-performance liquid chromatography (RP-HPLC) method was developed and validated for the simultaneous analysis of two drugs, levamisole hydrochloride (LH) and oxyclozanide (OX), in co-formulation for veterinary use. Materials and Methods: The new HPLC method was validated as per the ICH and other guidelines. A C18 column was used with a gradient program; eluent A was an equa Read More