Current Pharmaceutical Analysis - Volume 17, Issue 10, 2021

Background: A liquid chromatography-tandem mass spectrometric (LC-MS/MS) method had been developed for the quantification of acotiamide in rat plasma and been applied to pharmacokinetic studies. However, there was no LC-MS/MS method been developed for the determination of acotiamide in human plasma and its pharmacokinetic study. Objective: A simple and fast LC-MS/MS method was established and validate Read More

Background: Establishing R, S-enantiomer (S-KT and R-KT) chiral resolution and determination methods for KT are of great significance. Objective: This study aimed to establish a high-performance liquid chromatography (HPLC) method for the resolution and determination of ketorolac tromethamine (KT) enantiomers. Methods: A CHIRALPAK AGP column (0.4 × 10 cm, 5 μm) was used with 10 mmol/L ammonium acetate Read More

Aim: The aim is to develop and validate a single quantitative method for all DPP-IV inhibitors (Alogliptin, Vildagliptin, Sitagliptin, Saxagliptin, and Linagliptin). Background: Metformin is the most used antihyperglycemic drug in diabetes, therefore it is preferred to be combined with other classes of antihyperglycemic drugs, specially gliptins. Formulating these two drug classes in one pharmaceutical dosage form would be Read More

Background: Breast cancer is a heterogenic disease that comprises of various morphologies with intrinsic subtypes and is principally responsible for casualties among female cancer patients. Triple-negative breast cancer (TNBC) is the most aggressive subtype with a high probability of relapsing, hence successful treatment can be quite challenging. The pathogenesis of TNBC remains ambiguous and the identification of dependabl Read More

Background: Telmisartan and rosuvastatin calcium fixed-dose combination double-layer tablet is used for hypertension and hyperlipidemia treatment. Owing to the critical problem in establishing the specificity, precision, and accuracy of the USP analysis method and simultaneous analysis method for both components, an analysis method that could be applied to researchers in the field is urgently needed. Objective Read More

Background: Vildagliptin is a drug for the treatment of diabetes. DPP-IV inhibitor represents a new class of oral antihyperglycemic agents to treat patients with type 2 diabetes. Several RP-HPLC methods have been reported to determine Vildagliptin alone. However, it has been noted that there are no available stability-indicating methods in pharmacopeias (USP/BP/EP/JP) nor in the available literature to quantify known and unkn Read More

Background: Impurities in pharmaceutical compounds can influence their clinical effects and represent a potential health risk. To ensure the safety and effectiveness of a drug, it is necessary to investigate potential impurities. Methods: In this paper, a new impurity was separated and characterized by two-dimensional high performance liquid chromatography coupled to quadrupole time-of-flight tandem mass spectrom Read More

Background: Cardiovascular diseases (CVD) have been reported in 8%-16% of patients with 2019 coronavirus disease (COVID-19). Digoxin is one of the main drugs to treat CVD. Objective: The clinician conducted therapeutic drug monitoring (TDM) of digoxin according to the drug usage on patients to monitor the concentration of digoxin, so as to avoid its toxic and side effects, and provide a theoretical reference for clinical Read More

Background: There is no single chiral method for the quantitation of teneligliptin stereoisomers by high performance liquid chromatography (HPLC). Hence, there is a need for the quantification of teneligliptin (TNGP) and its stereoisomers. Objective: The main aim of the research work is to develop a novel simple, selective, precise and accurate HPLC method for the separation of TNGP and its stereoisomers. Methods: Differ Read More

Background: Thiazide diuretics are listed as one of the first-line antihypertensive treatment agents. Hydrochlorothiazide is mainly formulated with many antihypertensive agents as combined pill therapy. Objective: In this study, a new, simple, rapid and sensitive LC-MS/MS assay was developed, optimized and validated for the simultaneous quantification of commonly prescribed cardiovascular combined medications that are use Read More

Background: The reaction between N1-methylnicotinamide iodide (NMNI) and the active methylene group-containing compounds yields a fluorescent product, which can be used in the quantitative determination of certain drugs. Objective: The objective is to develop, adapt, and validate a simple spectrofluorometric method for the quantitative analysis of modafinil 1, budesonide 2, allicin 3, and florfenicol 4. Meth Read More