Skip to content
2000
  1. Home
  2. A-Z Publications
  3. Current Nutraceuticals
  4. Volume 2, Issue 2
  5. Article
Volume 2, Issue 2
  • ISSN: 2665-9786
  • E-ISSN: 2665-9794

Abstract

Eggshell membrane (ESM), a protein and collagen-rich membrane between the eggshell and egg white, has usually been regarded as waste and overlooked. However, its potential is now being highlighted in many industrial and technological applications. In the literature, natural bioactive compounds (NBCs) have been used together with various proteins.

Preparation and characterization methods of the eggshell and eggshell membrane are summarized

Various studies showed the utilization of eggshell membranes as an adsorbent, scaffold, wound dressing, and vascular graft. Due to the chemical composition of the eggshell membrane, its superior binding and adsorptive properties increase the stability of the bioactive compounds

This review focuses on the potential utilization of eggshell membranes as functional food and nutraceuticals.

© 2021 Bentham Science Publishers
Loading

Article metrics loading...

/content/journals/cnt/10.2174/2665978601999200727103101
2021-07-01
2025-01-10

  • From This Site

    /content/journals/cnt/10.2174/2665978601999200727103101
    dcterms_title,dcterms_subject,pub_keyword
    -contentType:Contributor -contentType:Concept -contentType:Institution
    10
    5
Loading full text...

Full text loading...

References

  1. MartosG. MolinaE. Lopez-Exposito. Eggs.Proteomics Foods- Princ Appl; Toldra, F. NolletL.M.L. 2005305322
     [Google Scholar]
  2. GautronJ. NysY. Eggshell Matrix Proteins.Bioact Egg Compd2007103108
     [Google Scholar]
  3. NysY. GautronJ. Garcia-RuizJ.M. HinckeM.T. Avian eggshell mineralization: Biochemical and functional characterization of matrix proteins.C. R. Palevol2004354956210.1016/j.crpv.2004.08.002
     [Google Scholar]
  4. BalážM. Eggshell membrane biomaterial as a platform for applications in materials science.Acta Biomater.20141093827384310.1016/j.actbio.2014.03.020 24681370
     [Google Scholar]
  5. WongM. HendrixM.J.C. von der MarkK. LittleC. SternR. Collagen in the egg shell membranes of the hen.Dev. Biol.19841041283610.1016/0012‑1606(84)90033‑2 6203793
     [Google Scholar]
  6. NakanoT. IkawaN.I. OzimekL. Chemical composition of chicken eggshell and shell membranes.Poult. Sci.200382351051410.1093/ps/82.3.510 12705414
     [Google Scholar]
  7. PanheleuxM. BainM. FernandezM.S. MoralesI. GautronJ. AriasJ.L. SolomonS.E. HinckeM. NysY. Organic matrix composition and ultrastructure of eggshell: a comparative study.Br. Poult. Sci.199940224025210.1080/00071669987665 10465392
     [Google Scholar]
  8. GolubkinaN.A. PapazyanT.T. Selenium distribution in eggs of avian species.Comp. Biochem. Physiol. B Biochem. Mol. Biol.20061453-438438810.1016/j.cbpb.2006.08.007 17055312
     [Google Scholar]
  9. SahM.K. RathS.N. Soluble eggshell membrane: A natural protein to improve the properties of biomaterials used for tissue engineering applications.Mater. Sci. Eng. C20166780782110.1016/j.msec.2016.05.005 27287179
     [Google Scholar]
  10. BalážM. ZorkovskáA. FabiánM. GirmanV. BriančinJ. Eggshell biomaterial: Characterization of nanophase and polymorphs after mechanical activation.Adv. Powder Technol.2015261597160810.1016/j.apt.2015.09.003
     [Google Scholar]
  11. YiF. GuoZ.X. ZhangL.X. YuJ. LiQ. Soluble eggshell membrane protein: preparation, characterization and biocompatibility.Biomaterials200425194591459910.1016/j.biomaterials.2003.11.052 15120504
     [Google Scholar]
  12. ZhaoY.H. ChiY.J. Characterization of collagen from eggshell membrane.Biotechnology (Faisalabad)2009825425810.3923/biotech.2009.254.258
     [Google Scholar]
  13. DeviP.S. BanerjeeS. ChowdhuryS.R. KumarG.S. Eggshell membrane: A natural biotemplate to synthesize fluorescent gold nanoparticles.RSC Advances20122115781158510.1039/c2ra21053c
     [Google Scholar]
  14. TsaiW.T. YangJ.M. LaiC.W. ChengY.H. LinC.C. YehC.W. Characterization and adsorption properties of eggshells and eggshell membrane.Bioresour. Technol.200697348849310.1016/j.biortech.2005.02.050 15896954
     [Google Scholar]
  15. SnyderT. Eggshell Membrane Separation Process, United States Patent No.9,370,778 B2., 2016
  16. AdamsR.G. FranklinM.R. Vacuum Treatment Of An Input Stream Without Runing Delicate Output Fractions United States Patent No. 7,017,277 B1.,2006
  17. VladV. Eggshell Membrane Separation Method United States Patent No. 7.584,909 B2., 2009
  18. ThoroskiJ.H. Eggshell processing methods and apparatus.United States2003
     [Google Scholar]
  19. YooS. HsiehJ.S. ZouP. KokoszkaJ. Utilization of calcium carbonate particles from eggshell waste as coating pigments for ink-jet printing paper.Bioresour. Technol.2009100246416642110.1016/j.biortech.2009.06.112 19665373
     [Google Scholar]
  20. HussainA. DevS. GariepyY. OrsatV. RaghavanG.S.V. Microwave-Assisted Separation Of Eggshell And Membrane Csbe100630-Presented atPostharvest Technology and Process Engineering Conference2009
     [Google Scholar]
  21. SuzukiFK WangP-Y WeatherspoonJ. MeadLC. Method of producing eggshell powder.2004
     [Google Scholar]
  22. NewL. Eggshell membrane separation process United States Patent No. 8,448,884 B2., 2013
  23. LongF.D. AdamsR.G. DeVoreD.P. Preparation of hyaluronic acid from eggshell membrane.United States2005
     [Google Scholar]
  24. SinghB.R. DeOliveiraD.B. FuF-N. FullerM.P. Fourier transform infrared analysis of amide III bands of proteins for the secondary structure estimation.United States199310.1117/12.145242
     [Google Scholar]
  25. BarthA. Infrared spectroscopy of proteins. Biochim. Biophys. Acta - Bioenerg.Elsevier200710731101
     [Google Scholar]
  26. Torres-MansillaA.C. Delgado-MejíaE. Influence of separation techniques with acid solutions on the composition of eggshell membrane.Int. J. Poult. Sci.20171645145610.3923/ijps.2017.451.456
     [Google Scholar]
  27. HsiehS. ChouH.H. HsiehC.W. WuD.C. KuoC.H. LinF.H. Hydrogen peroxide treatment of eggshell membrane to control porosity.Food Chem.201314132117212110.1016/j.foodchem.2013.04.115 23870936
     [Google Scholar]
  28. TorresF.G. TroncosoO.P. PiaggioF. HijarA. Structure-property relationships of a biopolymer network: the eggshell membrane.Acta Biomater.2010693687369310.1016/j.actbio.2010.03.014 20227532
     [Google Scholar]
  29. FreireM.N. HolandaJ.N.F. Characterization of avian eggshell waste aiming its use in a ceramic wall tile paste.Ceramica20065224024410.1590/S0366‑69132006000400004
     [Google Scholar]
  30. FattalE. RoquesB. PuisieuxF. Blanco-PrietoM.J. CouvreurP. Multiple emulsion technology for the design of microspheres containing peptides and oligopeptides.Adv. Drug Deliv. Rev.1997281859610.1016/S0169‑409X(97)00052‑5 10837566
     [Google Scholar]
  31. GuruP.S. DashS. Sorption on eggshell waste--a review on ultrastructure, biomineralization and other applications.Adv. Colloid Interface Sci.2014209496710.1016/j.cis.2013.12.013 24456801
     [Google Scholar]
  32. YiF. LuJ.W. GuoZ.X. YuJ. Mechanical properties and biocompatibility of soluble eggshell membrane protein/poly(vinyl alcohol) blend films.J. Biomater. Sci. Polym. Ed.20061791015102410.1163/156856206778365997 17094639
     [Google Scholar]
  33. YiF. YuJ. GuoZ.X. ZhangL.X. LiQ. Natural bioactive material: A preparation of soluble eggshell membrane protein.Macromol. Biosci.2003323423710.1002/mabi.200390030
     [Google Scholar]
  34. AhlbornG.J. ClareD.A. SheldonB.W. KellyR.W. Identification of eggshell membrane proteins and purification of ovotransferrin and β-NAGase from hen egg white.Protein J.2006251718110.1007/s10930‑006‑0010‑8 16721662
     [Google Scholar]
  35. TakahashiK. ShiraiK. KitamuraM. HattoriM. Soluble egg shell membrane protein as a regulating material for collagen matrix reconstruction.Biosci. Biotechnol. Biochem.19966081299130210.1271/bbb.60.1299 8987547
     [Google Scholar]
  36. KoseM.D. BayraktarO. Encapsulated Plant- Derived Polyphenols as Potential Cancer Treatment Agents.Herb Med Back to Futur Cancer Ther; Murad, F.; Rahman, A.; Bian, K.., Eds.; Bentham Science20199112210.2174/9789811411205119030005
     [Google Scholar]
  37. KöseM.D. BaşpınarY. BayraktarO. Electroencapsulation (Electrospraying & Electrospinning) of Active Compounds for Food Applications.Curr. Pharm. Des.201925161881188810.2174/1381612825666190717125538 31333111
     [Google Scholar]
  38. BaspinarY. ÜstündasM. BayraktarO. SezginC. Curcumin and piperine loaded zein-chitosan nanoparticles: Development and in-vitro characterisation.Saudi Pharm. J.201826332333410.1016/j.jsps.2018.01.010 29556123
     [Google Scholar]
  39. ColantuonoA. VitaglioneP. FerracaneR. CampanellaO.H. HamakerB.R. Development and functional characterization of new antioxidant dietary fibers from pomegranate, olive and artichoke by-products.Food Res. Int.201710115516410.1016/j.foodres.2017.09.001 28941678
     [Google Scholar]
  40. D’ArchivioM. FilesiC. Di BenedettoR. GargiuloR. GiovanniniC. MasellaR. Polyphenols, dietary sources and bioavailability.Ann. Ist. Super. Sanita2007434348361 18209268
     [Google Scholar]
  41. KöseM.D. BayraktarO. Encapsulation of lycopene using electrospraying method.Biointerface Res Appl Chem.2016610191025
     [Google Scholar]
  42. RyanL. PetitS. Addition of whole, semiskimmed, and skimmed bovine milk reduces the total antioxidant capacity of black tea.Nutr. Res.2010301142010.1016/j.nutres.2009.11.005 20116655
     [Google Scholar]
  43. von StaszewskiM. PilosofA.M.R. JagusR.J. Antioxidant and antimicrobial performance of different Argentinean green tea varieties as affected by whey proteins.Food Chem.201112518619210.1016/j.foodchem.2010.08.059
     [Google Scholar]
  44. HanJ. ChangY. BrittenM. St-GelaisD. ChampagneC.P. FustierP. Interactions of phenolic compounds with milk proteins.Eur. Food Res. Technol.20192451881188810.1007/s00217‑019‑03293‑1
     [Google Scholar]
  45. LabuckasD.O. MaestriD.M. PerellóM. MartínezM.L. LamarqueA.L. Phenolics from walnut (Juglans regia L.) kernels: Antioxidant activity and interactions with proteins.Food Chem.200810760761210.1016/j.foodchem.2007.08.051
     [Google Scholar]
  46. OzdalT. CapanogluE. AltayF. A review on protein-phenolic interactions and associated changes.Food Res. Int.20135195497010.1016/j.foodres.2013.02.009
     [Google Scholar]
  47. AliH. AlliI. IsmailA. KermashaS. Protein-phenolic interactions in food.Eurasian J Anal Chem.20127123133
     [Google Scholar]
  48. KöseM.D. BayraktarO. Natural Bioactive Compounds Loaded Eggshell Membrane Using Foam Separation.4th Int Clean Pers Care Prod Prod Technol Symp Exhib2019
     [Google Scholar]
  49. JensenG.S. ShahB. HoltzR. PatelA. LoD.C. Reduction of facial wrinkles by hydrolyzed water-soluble egg membrane associated with reduction of free radical stress and support of matrix production by dermal fibroblasts.Clin. Cosmet. Investig. Dermatol.2016935736610.2147/CCID.S111999 27789968
     [Google Scholar]
  50. YanS. XuY. LinY.J. ZhangZ. ZhangX. YilmazG. Ethanol-lubricated expanded-polytetrafluoroethylene vascular grafts loaded with eggshell membrane extract and heparin for rapid endothelialization and anticoagulation.Appl. Surf. Sci.202051114556510.1016/j.apsusc.2020.145565
     [Google Scholar]
  51. KumarA. KumarA. Fabrication of eggshell membrane-based novel buccal mucosa-mimetic surface and mucoadhesion testing of chitosan oligosaccharide films.J. Mater. Res.2019343777378610.1557/jmr.2019.318
     [Google Scholar]
  52. MotojiH. ToM. HidakaK. MatsuoM. Vitamin C and eggshell membrane facilitate orthodontic tooth movement and induce histological changes in the periodontal tissue.J Oral Biosci2020621808710.1016/j.job.2020.01.006 32032751
     [Google Scholar]
  53. Kobus-CisowskaJ. Szymanowska-PowałowskaD. Szymandera-BuszkaK. RezlerR. JarzębskiM. SzczepaniakO. MarciniakG. Jędrusek-GolińskaA. Kobus-MorysonM. Effect of fortification with calcium from eggshells on bioavailability, quality, and rheological characteristics of traditional Polish bread spread.J. Dairy Sci.2020103(8), 6918-6929. 32505401
     [Google Scholar]
  54. LiX. MaM. AhnD.U. HuangX. Preparation and characterization of novel eggshell membrane-chitosan blend films for potential wound-care dressing: From waste to medicinal products.Int. J. Biol. Macromol.201912347748410.1016/j.ijbiomac.2018.10.215 30412761
     [Google Scholar]
  55. LiX. CaiZ. AhnD.U. HuangX. Development of an antibacterial nanobiomaterial for wound-care based on the absorption of AgNPs on the eggshell membrane.Colloids Surf. B Biointerfaces201918311044910.1016/j.colsurfb.2019.110449 31465939
     [Google Scholar]
  56. HuangX. ChangL. LuY. LiZ. KangZ. ZhangX. LiuM. YangD.P. Plant-mediated synthesis of dual-functional Eggshell/Ag nanocomposites towards catalysis and antibacterial applications.Mater. Sci. Eng. C202011311101510.1016/j.msec.2020.111015 32487416
     [Google Scholar]
  57. SabuU. TripathiN. LogeshG. RashadM. JoyA. BalasubramanianM. Development of biomorphic C-ZnO with in situ formation of ZnS using eggshell membrane as bio-template.Ceram. Int.202010.1016/j.ceramint.2020.06.057
     [Google Scholar]
  58. AjisawaA. Dissolution aqueous of silk fibroin with calciumchloride/ethanol solution.J Sericultural Sci Japan [Internet]1997679194
     [Google Scholar]
  59. MohammadzadehL. RahbarghaziR. SalehiR. MahkamM. A novel egg-shell membrane based hybrid nanofibrous scaffold for cutaneous tissue engineering.J. Biol. Eng.2019137910.1186/s13036‑019‑0208‑x 31673286
     [Google Scholar]
  60. SantosK.O. BarbosaR.C. da Silva BuritiJ. BezerraA.G. Junior; de Sousa, W.J.B.; de Barros, S.M.C. Thermal, chemical, biological and mechanical properties of chitosan films with powder of eggshell membrane for biomedical applications.J. Therm. Anal. Calorim.201913672573510.1007/s10973‑018‑7666‑0
     [Google Scholar]
  61. FarjahG.H. HeshmatianB. KarimipourM. SaberiA. Using eggshell membrane as nerve guide channels in peripheral nerve regeneration.Iran. J. Basic Med. Sci.2013168901905 24106593
     [Google Scholar]
  62. DaengprokW. GarnjanagoonchornW. NaivikulO. PornsinlpatipP. IssigonisK. MineY. Chicken eggshell matrix proteins enhance calcium transport in the human intestinal epithelial cells, Caco-2.J. Agric. Food Chem.200351206056606110.1021/jf034261e 13129316
     [Google Scholar]
  63. JainS. AnalA.K. Preparation of eggshell membrane protein hydrolysates and culled banana resistant starch-based emulsions and evaluation of their stability and behavior in simulated gastrointestinal fluids.Food Res. Int.201810323424210.1016/j.foodres.2017.10.042 29389611
     [Google Scholar]
  64. LeeM.C. HuangY.C. Soluble eggshell membrane protein-loaded chitosan/fucoidan nanoparticles for treatment of defective intestinal epithelial cells.Int. J. Biol. Macromol.201913194995810.1016/j.ijbiomac.2019.03.113 30910672
     [Google Scholar]
  65. TembeS. KubalB.S. KarveM. D’SouzaS.F. Glutaraldehyde activated eggshell membrane for immobilization of tyrosinase from Amorphophallus companulatus: application in construction of electrochemical biosensor for dopamine.Anal. Chim. Acta2008612221221710.1016/j.aca.2008.02.031 18358868
     [Google Scholar]
  66. YangJ.Y. ChuangS.S. YangW.G. TsayP.K. Egg membrane as a new biological dressing in split-thickness skin graft donor sites: a preliminary clinical evaluation.Chang Gung Med. J.200326315315912790218
     [Google Scholar]
  67. YooJ.H. KimJ.K. YangH.J. ParkK.M. Effects of egg shell membrane hydrolysates on UVB-radiation-induced wrinkle formation in SKH-1 hairless mice.Han-gug Chugsan Sigpum Hag-hoeji2015351587010.5851/kosfa.2015.35.1.58 26761801
     [Google Scholar]
  68. King’oriA.M. A Review of the uses of poultry eggshells and shell membranes.Int. J. Poult. Sci.20111090891210.3923/ijps.2011.908.912
     [Google Scholar]
  69. FrancisA.A. Abdel RahmanM.K. The environmental sustainability of calcined calcium phosphates production from the milling of eggshell wastes and phosphoric acid.J. Clean. Prod.20161371432143810.1016/j.jclepro.2016.08.029
     [Google Scholar]
  70. AmaralM.C. SiqueiraF.B. DestefaniA.Z. HolandaJ.N.F. Soil-cement bricks incorporated with eggshell waste.Proc Inst Civ Eng Waste Resour Manag.2013166137141
     [Google Scholar]
  71. PanditP.R. FulekarM.H. Egg shell waste as heterogeneous nanocatalyst for biodiesel production: Optimized by response surface methodology.J. Environ. Manage.2017198Pt 131932910.1016/j.jenvman.2017.04.100 28494420
     [Google Scholar]
  72. ChoudharyR. KoppalaS. SwamiappanS. Bioactivity studies of calcium magnesium silicate prepared from eggshell waste by sol-gel combustion synthesis.J Asian Ceram Soc.2015317317710.1016/j.jascer.2015.01.002
     [Google Scholar]
  73. QuinaM.J. SoaresM.A.R. Quinta-FerreiraR. Applications of industrial eggshell as a valuable anthropogenic resource.Resour. Conserv. Recycling201712317618610.1016/j.resconrec.2016.09.027
     [Google Scholar]
  74. GirelliA.M. AstolfiM.L. ScutoF.R. Agro-industrial wastes as potential carriers for enzyme immobilization: A review.Chemosphere202024412536810.1016/j.chemosphere.2019.125368 3179099
     [Google Scholar]
/content/journals/cnt/10.2174/2665978601999200727103101
Loading
Natural Bioactive Compounds Loaded Eggshell Membrane from Waste Food Industry: Preparation and Characterization
Current Nutraceuticals 2, 106 (2021); https://doi.org/10.2174/2665978601999200727103101
/content/journals/cnt/10.2174/2665978601999200727103101
/content/journals/cnt/10.2174/2665978601999200727103101
Loading

Data & Media loading...


  • Article Type:     Review Article
Keyword(s): Biomaterial; biopolymers; health applications; protein-polyphenol interactions; utilization; value-added products; waste

Most Cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error
Please enter a valid_number test