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Volume 1, Issue 1
  • ISSN: 2665-9786
  • E-ISSN: 2665-9794

Abstract

The essential trace element and micronutrient selenium exerts most of its biological actions through incorporation into selenoproteins as selenocysteine. Two further types of Se-containing proteins exist, including those that have selenomethionine incorporated instead of methionine, and the group of selenium-binding proteins. We previously described an ortholog of selenium-binding protein 1 (SELENBP1) in the nematode , Y37A1B.5, and demonstrated that it confers resistance to toxic selenite concentrations while impairing general stress resistance and life expectancy of .

We tested for the effect of selenite on expression, and we analyzed whether also shows a lifespan-modulating effect when the nematodes are deficient in the selenoenzyme thioredoxin reductase-1 (TRXR-1).

expressing a translational reporter construct encoding GFP-tagged Y37A1B.5 under the control of the promoter were exposed to selenite, followed by fluorescence microscopic analysis of GFP levels. Lifespan analyses and RNA interference experiments were performed in -deficient worms.

We here demonstrate that selenite at toxic concentrations stimulates the expression of the translational reporter. The lifespan-extending effect of deficiency was preserved upon the deletion of the only selenoprotein in , TRXR-1.

These data suggest that (1) Y37A1B.5 may serve as a selenite-responsive buffer against high environmental selenium concentrations and that (2) lifespan extension elicited by knockdown does not require functional TRXR-1.

© 2020 Lars-Oliver Klotz
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2020-05-01
2024-11-22

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/content/journals/cnt/10.2174/2665978601666200212105825
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Selenite-Induced Expression of a Caenorhabditis elegans Pro-Aging Factor and Ortholog of Human Selenium-Binding Protein 1
Current Nutraceuticals 1, 73; https://doi.org/10.2174/2665978601666200212105825
/content/journals/cnt/10.2174/2665978601666200212105825
/content/journals/cnt/10.2174/2665978601666200212105825
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  • Article Type:     Research Article
Keyword(s): Caenorhabditis elegans; lifespan; selenite; Selenium-binding protein; stress signaling; thioredoxin reductase

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