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Volume 2, Issue 1
  • ISSN: 2666-0016
  • E-ISSN: 2666-0008

Abstract

Cilnidipine and Metoprolol Succinate are antihypertensive agents used in the treatment of hypertension. Methods: A pharmacokinetic study of Cilnidipine and Metoprolol Succinate in rat plasma was carried out using the chromatographic method.

The chromatographic method involved a reverse phase C18 column, using acetonitrile and pH 4.0 water in the ratio of 80:20 v/v as mobile phase, a flow rate of 1.2 ml/min, and UV detection at 231 nm. pharmacokinetic studies were performed on rats. Rats were treated with Cilnidipine (1mg/kg) and Metoprolol Succinate (1 mg/kg) orally, and blood samples were collected at 0, 0.5, 1, 2, 4, 6, 8, 12 and 24 h post-treatment.

The retention time of Plasma, Cilnidipine and Metoprolol Succinate was found to be 2.3, 3.1 and 5.5 min, respectively. Linearity was acceptable in the concentration range of 2-10 and 10-50 for Cilnidipine and Metoprolol Succinate, respectively. The intra-day and inter-day variances were found to be less than 2. The mean recovery of Cilnidipine and Metoprolol Succinate was 100.12 and 100.15, respectively. The assay was successfully applied to a pharmacokinetic study in rat after oral administration. After oral administration, maximal concentration (Cmax) of Cilnidipine and Metoprolol Succinate was found to be 460.01 and 642.13 (μg g/mL), and the half-life was found at 2.0 and 3.904 hours, respectively.

The present method was successfully applied to the pharmacokinetic study of Cilnidipine and Metoprolol Succinate in rat plasma after oral administration.

© 2022 Bentham Science Publishers
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2021-06-16
2025-03-17

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References

  1. ValicM.S. HalimM. SchimmerP. ZhengG. Guidelines for the experimental design of pharmacokinetic studies with nanomaterials in preclinical animal models.J. Control. Release20203238310110.1016/j.jconrel.2020.04.002 32278829
     [Google Scholar]
  2. SicaD.A. Rationale for fixed-dose combinations in the treatment of hypertension: the cycle repeats.Drugs200262344346210.2165/00003495‑200262030‑00003 11827559
     [Google Scholar]
  3. The Merck Index, An Encyclopedia of Chemicals, Drugs, and Biologicals, Merck Reasearch Laboratories; 2006, 493, p. 6149.
  4. ShinjiT. DenanJ. ShizukaA. KazumiN. MizuoM. Powerful vascular protection by combining cilnidipine with valsartan in stroke-prone, spontaneously hypertensive rats.Hypertens. Res.20133634234810.1038/hr.2012.187
     [Google Scholar]
  5. BruntonL.L. Hilal-DandanR. KnollmannB.C. Goodman, and Gilman’s The pharmacology basis of therapeutics. Medical publishing Division: New York, 2001, pp. 249-260.
  6. British pharmacopoeia, Vol. I and II, 2009, pp. 3933-3042.
  7. United State Pharmacopoeia 30- National Formulary, 2007, 3(25), Asian edition, United State Pharmacopoeial Convention, Inc; 2647.
  8. KarioK. NariyamaJ. KidoH. AndoS. TakiuchiS. EguchiK. NiijimaY. AndoT. NodaM. Effect of a novel calcium channel blocker on abnormal nocturnal blood pressure in hypertensive patients.J. Clin. Hypertens. (Greenwich)201315746547210.1111/jch.12113 23815534
     [Google Scholar]
  9. MinamiJ. IshimitsuT. KawanoY. NumabeA. MatsuokaH. Comparison of 24-hour blood pressure, heart rate, and autonomic nerve activity in hypertensive patients treated with cilnidipine or nifedipine retard.J. Cardiovasc. Pharmacol.199832233133610.1097/00005344‑199808000‑00023 9700998
     [Google Scholar]
  10. LeeH.W. SeoJ.H. LeeH.S. JeongS.Y. ChoY.W. LeeK.T. Development of a liquid chromatography/negative-ion electrospray tandem mass spectrometry assay for the determination of cilnidipine in human plasma and its application to a bioequivalence study.J. Chromatogr. B Analyt. Technol. Biomed. Life Sci.20088621-224625110.1016/j.jchromb.2007.11.016 18155975
     [Google Scholar]
  11. XianhuaZ. SuodiZ. RongshengZ. JinO. LiX. WillyR.G. Baeyens, Determination of cilnidipine, a new calcium antagonist, in human plasma using high performance liquid Chromatography with tandem mass spectrometric detection.Analytica chemica acta2007600142-14610.1016/j.aca.2006.11.072
     [Google Scholar]
  12. PawarP. GandhiS.V. DeshpandeP.B. VanjariS. ShelarS.U. Simultaneous RP-HPLC estimation of cilnidipine and telmisartan in combined tablet dosage form.Pelagia Research Library Der Chemica Sinica201342610
     [Google Scholar]
  13. MinamiJ. KawanoY. MakinoY. MatsuokaH. TakishitaS. Effects of cilnidipine, a novel dihydropyridine calcium antagonist, on autonomic function, ambulatory blood pressure and heart rate in patients with essential hypertension.Br. J. Clin. Pharmacol.200050661562010.1046/j.1365‑2125.2000.00299.x 11136301
     [Google Scholar]
  14. AqilM. AliA. AhadM. SultanaY. NajmiA.K. Validated HPLC method for estimation of Metoprolol in Human plasma.Acta Chromatogr.200719130140
     [Google Scholar]
  15. Prasada RaoCHMM RahamanSA Ragjendra PrasadY Gangi Reddy, P RP-HPLC method of simultaneous estimation of Amlodipine Besylate and metoprolol in combined dosage form.International journal of pharmaceutical research and development20102969-76
     [Google Scholar]
  16. GosaiM. TannaR. ThumarK. ChikhaliaJ. RP-HPLC method for simultaneous estimation of Metoprolol succinate and Clopidogrel bisulphate from tablet dosage. Inventi Rapid: Pharm Analysis &.Qual. Assur.2012317
     [Google Scholar]
  17. Chandra BoseR.J. SivanseyaG. Krishna kumar, D. Validated RP-HPLC method for the simultaneous estimation of ramipril and metoprolol tartrate in bulk and tablet dosage form.Asian J. Biochem. Pharm. Res.201112171177
     [Google Scholar]
  18. Bhargavi DurgaK Naga MounikaI ShajhanSK Srinivasa rao, N; Sarath, Chandra RP- HPLC method for estimation of Metaprolol in bulk drug.International journal of pharmaceutical research and development201212151-157
     [Google Scholar]
  19. U.S. Department of Health and Human Services, Food and Drug Administration: In: Bioanalytical Method Validation, Guidance for Industry; 2013
/content/journals/ccchem/10.2174/2210676611666210616142035
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Bioanalytical Method Development and Validation of Cilnidipine and Metoprolol Succinate by RP-HPLC: Its Pharmacokinetic Application
Curr Chin Chem 2, E160621194113 (2022); https://doi.org/10.2174/2210676611666210616142035
/content/journals/ccchem/10.2174/2210676611666210616142035
/content/journals/ccchem/10.2174/2210676611666210616142035
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  • Article Type:     Research Article
Keyword(s): Cilnidipine; metoprolol succinate; pharmacokinetic study; rat plasma; RP-HPLC; validation of cilnidipine

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