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- Volume 25, Issue 11, 2018
Protein and Peptide Letters - Volume 25, Issue 11, 2018
Volume 25, Issue 11, 2018
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Structural Insights on the Obscurin-Binding Domains in Titin
Authors: Allyn G. Letourneau and Nathan T. WrightIntroduction: The giant muscular proteins titin and obscurin bind to each other at the Zdisk during muscle development. This binding event is mediated through two domains from each protein: ZIg9/10 from titin and Ig58/59 from obscurin. This interaction helps stabilize and organize the sarcomere; ablation of this binding leads to muscular dystrophy. Objective: Here we solve the high-resolution solution structure of titin ZI Read More
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Efficient Expression and Purification of Cryptochrome1 from Columbia livia in E. coli
Authors: Xiaoxia Yuan, Wenhao Wang, Wenjian Wu and Jing WangBackground: Cryptochrome is a flavin-binding blue-light photoreceptor that functions in growth and development in plants, the circadian clock in animals and navigation in birds. However, a lack of purified cryptochrome has hindered studies of the structure and function of this protein. In this study, we obtained a substantial amount of the Columbia livia Cryptochrome1 (ClCry1) protein by using a prokaryotic expression system. Read More
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Expression and Purification of Tag-removed Human IL37 by Digestion on Beads in Escherichia coli
Authors: Daichao Wu, Guoqing Li, Yao Ma, Jin Liu, Yukun Li, Juan Wang, Junhui Bai, Sijie Tan, Meixiang Li and Yongheng ChenBackground: Human Interleukin 37 (IL37), a unique anti-inflammatory cytokine of IL1 family member, plays critical roles in innate and adaptive immunity and inflammation. Objective: Preparation of high purity and tag-removed recombinant IL37 protein (rIL37) is critical for its clinical application. Method: In this study, we constructed an N-terminal cleavable GST-fused IL37 expression vector for recombinant expression Read More
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Purification and Characterization of a Thermostable Cellobiohydrolase from Thermotoga petrophila
Background: Cellulose, being the most abundant biopolymer found in nature, can be utilized for bioethanol production to cater the future energy needs. Due to increased usage of fossil fuel it has been predicted that fossil fuel reserves may be depleted by year 2050. These concerns need serious attention and focus should be diverted to renewable fuels that are based on natural resources. Cellulases including exoglucanas Read More
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Classification-based QSAR Models for the Prediction of the Bioactivity of ACE-inhibitor Peptides
Background: Local classification models were used to establish Quantitative Structure− Activity Relationships (QSARs) of bioactive di−, tri− and tetrapeptides, with their capacity to inhibit Angiotensin Converting Enzyme (ACE). These discrete models can thus predict this activity for other peptides obtained from functional foods. These types of peptides allow some foods to be considered nutraceuticals. Method: A database Read More
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Immunodominant IgE Epitopes of Der p 5 Allergen
Background: Der p 5 is an important allergen of Dermatophagoides pteronyssinus that plays a key role in allergic airway diseases. Its three dimensional structure (PDB 3MQ1) consists of three anti-parallel α-helices arranged in a helical bundle. Aggregation of Der p5 can modulate its allergenicity. This study aimed to identify the key residues of IgE binding epitopes of Der p 5. Methods: IgE binding epitopes of Der p 5 w Read More
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Volumes & issues
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Volume 32 (2025)
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Volume 31 (2024)
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Volume 30 (2023)
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Volume 29 (2022)
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Volume 28 (2021)
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Volume 27 (2020)
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Volume 26 (2019)
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Volume 25 (2018)
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Volume 24 (2017)
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Volume 23 (2016)
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Volume 22 (2015)
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Volume 21 (2014)
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Volume 20 (2013)
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Volume 19 (2012)
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Volume 18 (2011)
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Volume 17 (2010)
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Volume 16 (2009)
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Volume 15 (2008)
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Volume 14 (2007)
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Volume 13 (2006)
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Volume 12 (2005)
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Volume 11 (2004)
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Volume 10 (2003)
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Volume 9 (2002)
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Volume 8 (2001)
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