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image of Development and Validation of a High-Performance Thin-Layer Chromatography Method for Qualitative and Quantitative Analysis of Wogonin in S. barbata D. Don Herb

Abstract

Background and Aims

D. Don, a perennial herb with a rich history in traditional medicine, is known for its bioactive compounds, including wogonin. It possesses anti-inflammatory, antioxidant, and anticancer properties. Together with other bioactive compounds, wogonin contributes to the pharmacological activities associated with D. Don, making it a subject of interest in both traditional and modern medicinal research. This study aimed to develop and validate a High-Performance Thin-Layer Chromatography (HPTLC) method for the precise and accurate quantification of wogonin in the hydroethanolic extract of D. Don.

Methods

The process was developed and underwent rigorous validation following the ICH guidelines. Parameters such as linearity, specificity, instrument precision, precision, accuracy, limit of detection (LOD), limit of quantification (LOQ), and robustness were systematically evaluated to ensure the method's reliability.

Results

The HPTLC separation was conducted on TLC aluminum plates precoated with silica gel 60 F, and optimal separation was achieved using Ethyl acetate: toluene: formic acid (5: 4.9: 0.1, (v/v/v/v)) as the mobile phase. Densitometric scanning at 254 nm in reflection/absorbance mode revealed compact spots at Rf 0.87, corresponding to wogonin. Linear regression analysis demonstrated a robust linear relationship between peak area and the amount of wogonin in the range of 10-40 (µg/mg). The quantification of wogonin in crude extracts obtained was 3.274 µg/mg of extract.

Conclusion

In summary, the developed HPTLC method emerges as a straightforward, precise, and accurate tool for the rapid screening of active constituents, specifically wogonin, within the petroleum ether extracts of D. Don. This method holds promise for routine quality control and analysis of herbal materials and formulations containing D. Don, contributing to the ongoing exploration of its therapeutic potential.

© 2024 Bentham Science Publishers
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2024-11-11
2024-12-30

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/content/journals/cnt/10.2174/0126659786313702241010052103
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Development and Validation of a High-Performance Thin-Layer Chromatography Method for Qualitative and Quantitative Analysis of Wogonin in S. barbata D. Don Herb
Bentham Science Publishers ; https://doi.org/10.2174/0126659786313702241010052103
/content/journals/cnt/10.2174/0126659786313702241010052103
/content/journals/cnt/10.2174/0126659786313702241010052103
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  • Article Type:     Research Article
Keywords: Quantitative analysis ; Wogonin ; HPTLC ; Scutellaria barbata D. Don ; Validation of method

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