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Volume 2, Issue 1
  • ISSN: 2210-299X
  • E-ISSN: 2210-3007
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Abstract

Background

L., commonly known as Karonda, is renowned for its distinctive tangy flavor, making it a valuable ingredient in culinary applications. Additionally, its extensive array of phytoconstituents renders it a significant component of traditional Indian herbal medicine, where it has been used to address various health conditions. Despite its availability, palatability, and diverse phytochemical profile, Karonda remains under utilized, and lacks substantial scientific validation. Further research is needed to substantiate its therapeutic potential and elevate its status in modern scientific and medical contexts.

Methods

An ethanolic extract was prepared from the ripe fruits using a hot continuous extraction method. Phytochemical screening of the extract identified the presence of tannins, flavonoids, phenolic compounds, saponins, steroids, and carbohydrates. Subsequent analysis using spectrometric and spectro chromatographic techniques, including NMR, FTIR, and LC-MS, were conducted, supplemented by chromatographic studies, to further elucidate the extract's chemical profile and validate the presence of these bioactive compounds.

Results

The analysis of the ripe fruit extract identified several functional groups, including O-H, N-H, C=O, C-N, C-H, and –COOH, based on IR absorption bands observed in the high wave region at 3850 cm−1 and 1728 cm−1. Active compounds were further characterized by comparing these findings with standard reference charts. Proton environments and their electronic states were examined using 1H-NMR spectroscopy. The 1H-NMR spectrum revealed signals at specific δ ppm values corresponding to R-CH, R-CH-R, RO-C-H, and F-C-H groups, as detailed in the accompanying table.

Conclusion

These results provide a comprehensive chemical fingerprint of CA fruits offering scientific validation of its chemical nature and supporting its potential therapeutic applications.

© 2024 The Author(s). Published by Bentham Science Publisher.
This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: https://creativecommons.org/licenses/by/4.0/legalcode. This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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2024-11-14
2025-03-01

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References

  1. HaslerC.M. BlumbergJ.B. Phytochemicals: Biochemistry and physiology. Introduction.J Nutr.19991293756S757S10.1093/jn/129.3.756S.
     [Google Scholar]
  2. GibsonE.L. WardleJ. WattsC.J. Fruit and vegetable consumption, nutritional knowledge and beliefs in mothers and children.Appetite199831220522810.1006/appe.1998.01809792733
     [Google Scholar]
  3. MathaiK. Nutrition in the adult’s years in krauses food, nutrition and diet therapy.2000274275
     [Google Scholar]
  4. HarborneJ.B. Photochemical screening method, a guide to modern techniques of plant analysisChapman & Hall1998
     [Google Scholar]
  5. KhandelwalK.R. Practical pharmacognosy: Techniques and experiments.PuneNirali Prakasan2003160149156
     [Google Scholar]
  6. RaoBayya Subba An overview of general morphology assay method in Indian pharmacopeia.20148387
     [Google Scholar]
  7. AnonPharmacopoeia of India, The Indian Pharmacopoeia the Pharma review .New DelhiMinistry of Health and Family Welfare1996
     [Google Scholar]
  8. KadamV.B. MominR.K. WadikarM.S. AndhaleS.B. determination of acid insoluble ash values in some medicinal plants of genus secbania.J Biomed Pharm Res201323134
     [Google Scholar]
  9. AvnetGupta. Ash value and extractive value comparison between pet ether and ethanolic extracts of Bacopa monnieri, Evolvulus alsinoides , and Tinospora cordifolia .J Pharm Res2018124
     [Google Scholar]
  10. ViditaV. SalujaA.K. DholwaniK.K. Detection of heavy metal contents and proximate analysis of roots of Anogeissus latifolia .J Pharmacogn Phytochem201316
     [Google Scholar]
  11. PatilR.P. PaiS.R. PawarN.V. Chemical characterization, mineral analysis, and antioxidant potential of two underutilized berries (Carissa carandus and Eleagnus conferta ) from the Western Ghats of India.Crit Rev Food Sci Nutr20125243122010.1080/10408398.2010.500227.
     [Google Scholar]
  12. KokateC.K. Practical Pharmacognosy2005
     [Google Scholar]
  13. PoonamR.G JadhaoK.D. Evaluation of phenolic compound from spines and flowers of Bombax ceiba Linn.J Global Biosci5642444249
     [Google Scholar]
  14. VijayD.T BhambarR.S Estimation of total phenol, tannin, alkaloid and flavonoid in Hibiscus tiliaceus Linn. Wood Extracts.J Pharmacogn Phytochem2014
     [Google Scholar]
  15. SaeedN. KhanM.R. ShabbirM. Antioxidant activity, total phenolic and total flavonoid contents of whole plant extracts Torilis leptophylla L.BMC Complement Altern Med20121222110.1186/1472‑6882‑12‑221.
     [Google Scholar]
  16. KellerA. NesvizhskiiA.I. KolkerE. AebersoldR. Empirical statistical model to estimate the accuracy of peptide identifications made by MS/MS and database search.Anal. Chem.200274205383539210.1021/ac025747h12403597
     [Google Scholar]
  17. YatesJ.R.III Mass spectral analysis in proteomics.Annu. Rev. Biophys. Biomol. Struct.200433129731610.1146/annurev.biophys.33.111502.08253815139815
     [Google Scholar]
  18. KelleherN.L. NicewongerR.B. BegleyT.P. McLaffertyF.W. Identification of modification sites in large biomolecules by stable isotope labeling and tandem high resolution mass spectrometry. I.J. Biol. Chem.199727251322153222010.1074/jbc.272.51.322159405424
     [Google Scholar]
  19. FavrettoD. TraldiP. MS/MS applications in biological problems.Mass Spectrom. Rev.1993125-631339510.1002/mas.1280120504
     [Google Scholar]
  20. AzizN. KhanM.N. AliA. KhadimA. MuhsinahA.B. UddinJ. MusharrafS.G. Rapid analysis of flavonoids based on spectral library development in positive ionization mode using LC-HR-ESI-MS/MS.Arab. J. Chem.202215410373410.1016/j.arabjc.2022.103734
     [Google Scholar]
  21. CuyckensF. ClaeysM. Mass spectrometry in the structural analysis of flavonoids.J. Mass Spectrom.200439111510.1002/jms.58514760608
     [Google Scholar]
  22. KachlickiP. PiaseckaA. StobieckiM. MarczakŁ. Structural characterization of flavonoid glycoconjugates and their derivatives with mass spectrometric techniques.Molecule20162111149410.3390/molecules21111494.
     [Google Scholar]
  23. López-FernándezO. DomínguezR. PateiroM. MunekataP.E.S. RocchettiG. LorenzoJ.M. Determination of polyphenols using liquid chromatography-Tandem mass spectrometry technique (LC-MS/MS): A review.Antioxidants (Basel)20209647910.3390/antiox9060479.
     [Google Scholar]
/content/journals/cis/10.2174/012210299X320156240926044625
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Physicochemical, Phytochemical, NMR, FTIR and LC-MS Analysis of Extract of Ripe Fruits of Carissa carandus L.
Curr. Indian Sci. 2, e2210299X320156 (2024); https://doi.org/10.2174/012210299X320156240926044625
/content/journals/cis/10.2174/012210299X320156240926044625
/content/journals/cis/10.2174/012210299X320156240926044625
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  • Article Type:     Research Article
Keyword(s): Carissa carandus L.; Flavonoids; Fourier transform spectroscopy; Functional groups; Nuclear magnetic resonance; Phytochemical screening; Phytochemicals

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