Protein and Peptide Letters - Volume 29, Issue 9, 2022

Bacillus subtilis is a Gram-positive bacterium that has gained an unprecedented reputation as an expression system at the industrial scale due to characteristics, such as GRAS (Generally recognized as safe), ease of genetic manipulation, high growth rate on the cheap substrate, and short fermentation cycle. This expression system has been widely accepted for the production of various chemicals, pharmaceutical products, Read More

Background: Tyrosinase inhibitor developments have been widely attended by investigators for their various applications. Objective: A combination of virtual screening of docking simulations and biochemical inhibition kinetics was performed to find a new inhibitor of tyrosinase for the clinical application of an antipigment agent. Methods: We conducted docking simulations to detect tyrosinase key binding residues and used the Read More

Background: This study aimed to investigate the effects of irisin on rat tracheal smooth muscle contraction-relaxation responses and the roles of voltage-gated potassium (KV) channels, ATP-sensitive potassium (KATP) channels, and large-conductance calcium-activated potassium (BKCa) channels in these effects. Methods: Isometric contraction and relaxation responses of tracheal segments were measured using the tissu Read More

Introduction: Protein S-nitrosylation (SNO) and O-GlcNAcylation are important posttranslational modifications. The biological connection between SNO and O-GlcNAcylation is not clear. Objective: We aim to identify the crosstalk between SNO and O-GlcNAcylation during heat-shock. Methods: Ex vivo heat-shock on mouse tissues together with in vitro heat-shock on culture cells was performed and global levels of SNO and O-GlcNA Read More

Background: Lectins are proteins with therapeutic and diagnostic potential that can be applied in battling various ailments. Aim and Objective: This study was designed to purify and characterize the hemagglutinating activity derived from the leaves of Calotropis procera and its possible role in protecting the stomach against ethanol-induced lesions. Methods: The Calotropis procera leaf lectin (ProLec), was isolated by homogeni Read More

Background: Oral squamous cell carcinoma (OSCC) is one of the commonest malignancies of the oral cavity. FOXN3 is a tumor suppressor that represses the progression of many tumors. Nonetheless, its role in OSCC has not been elucidated. This work is performed to probe the role and dysregulation mechanism of FOXN3 in OSCC. Methods: FOXN3 mRNA and miR-299-5p expressions were quantified by quantitative real-ti Read More

Objective: To probe the role of circular RNA_0008768 (circ_0008768) in the development of pancreatic cancer (PC) and its regulatory mechanism. Methods: The expression levels of circ_0008768, miR-330-3p, and PTEN mRNA in PC tissues and cells were detected using qRT-PCR. Cell proliferation, migration and invasion of PC cells were detected by CCK-8 method, EdU method, and Transwell assay. The targeting relatio Read More

Background: Annexin V, a member of calcium-dependent phospholipid-binding proteins, selectively binds to the exposed phosphatidylserine, which can be used for in vitro apoptosis detection. Simultaneous staining of cells with annexin V-fluorescein isothiocyanate (FITC) and the non-vital dye propidium iodide (PI) enables the detection of apoptotic and necrotic cells. Objective: Our study aimed to express, purify, and stabilize th Read More