Full text loading...
- ISSN: 0929-8665
- E-ISSN: 1875-5305
-
Purification and Kinetics of Bovine Kidney Cortex Glutathione Reductase
- Source: Protein and Peptide Letters, Volume 17, Issue 5, May 2010, p. 667 - 674
-
- 01 May 2010
Abstract
Glutathione reductase was purified 34806-fold with a final yield of 85 % from the bovine kidney cortex. Some molecular and kinetic properties of purified enzyme are investigated. Product inhibition studies showed that the enzyme obeys ‘branched’ mechanism: KmNADPH 18 ± 3 μM and KmGSSG 65 ± 5 μM were determined.
© Bentham Science Publishers