Cloning and Characterization of a cDNA Encoding a Cowpea Seed Defensin and Analysis of its Expression

Andre O. Carvalho; Goncalo A. S. Filho; Beatriz S. Ferreira; Alan T. Branco; Anna L. Okorokova-Facanha; Valdirene M. Gomes

doi:10.2174/092986606778777515

ISSN: 0929-8665
E-ISSN: 1875-5305

Cloning and Characterization of a cDNA Encoding a Cowpea Seed Defensin and Analysis of its Expression
Authors: Andre O. Carvalho¹, Goncalo A. S. Filho², Beatriz S. Ferreira³, Alan T. Branco⁴, Anna L. Okorokova-Facanha⁵ and Valdirene M. Gomes⁶
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¹ Av. Alberto Lamego 2000,Laboratorio de Fisiologia e Bioquimica de Microrganismos, Centro de Biocienciase Biotecnologia/ Universidade Estadual do Norte Fluminense Darcy Ribeiro, Parque California, Campos dos Goytacazes - RJ, Brazil, CEP 28013-602. ² Av. Alberto Lamego 2000,Laboratorio de Fisiologia e Bioquimica de Microrganismos, Centro de Biocienciase Biotecnologia/ Universidade Estadual do Norte Fluminense Darcy Ribeiro, Parque California, Campos dos Goytacazes - RJ, Brazil, CEP 28013-602. ³ Av. Alberto Lamego 2000,Laboratorio de Fisiologia e Bioquimica de Microrganismos, Centro de Biocienciase Biotecnologia/ Universidade Estadual do Norte Fluminense Darcy Ribeiro, Parque California, Campos dos Goytacazes - RJ, Brazil, CEP 28013-602. ⁴ Av. Alberto Lamego 2000,Laboratorio de Fisiologia e Bioquimica de Microrganismos, Centro de Biocienciase Biotecnologia/ Universidade Estadual do Norte Fluminense Darcy Ribeiro, Parque California, Campos dos Goytacazes - RJ, Brazil, CEP 28013-602. ⁵ Av. Alberto Lamego 2000,Laboratorio de Fisiologia e Bioquimica de Microrganismos, Centro de Biocienciase Biotecnologia/ Universidade Estadual do Norte Fluminense Darcy Ribeiro, Parque California, Campos dos Goytacazes - RJ, Brazil, CEP 28013-602. ⁶ Av. Alberto Lamego 2000,Laboratorio de Fisiologia e Bioquimica de Microrganismos, Centro de Biocienciase Biotecnologia/ Universidade Estadual do Norte Fluminense Darcy Ribeiro, Parque California, Campos dos Goytacazes - RJ, Brazil, CEP 28013-602.
Source: Protein and Peptide Letters, Volume 13, Issue 10, Oct 2006, p. 1029 - 1036
DOI: https://doi.org/10.2174/092986606778777515
- Available online: 01 Oct 2006

Abstract

We have previously isolated a cowpea seed defensin with potent antifungal activity. Here we report the cloning of its cDNA obtained from RT-PCR. The sequence of the deduced protein VUDEF exhibited homology to plant defensins. Northern analysis revealed that VUDEF mRNA is accumulated during cowpea seed development and its level increases in seedling tissues after exposure to fungal pathogen and cold.

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2006-10-01

2025-05-25

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Article Type: Research Article

Keyword(s): Antifungal peptide; Fusarium oxysporum f. sp. phaseolus; phylogenetic relationship; seedling; Vigna unguiculata

Cloning and Characterization of a cDNA Encoding a Cowpea Seed Defensin and Analysis of its Expression

Abstract

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