Expression of the Canavalia brasiliensis Lectin (ConBr) in Tobacco Plants

Cristina Paiva da Silveira Carvalho; Tuana Oliveira Correia; Sandra Mara Serafim Ribeiro; Ingrid Cavalcante Silva; Jose Tadeu Abreu de Oliveira; Thalles Barbosa Grangeiro

doi:10.2174/092986606778777434

ISSN: 0929-8665
E-ISSN: 1875-5305

Expression of the Canavalia brasiliensis Lectin (ConBr) in Tobacco Plants
Authors: Cristina Paiva da Silveira Carvalho¹, Tuana Oliveira Correia², Sandra Mara Serafim Ribeiro³, Ingrid Cavalcante Silva⁴, Jose Tadeu Abreu de Oliveira⁵ and Thalles Barbosa Grangeiro⁶
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¹ Laboratorio de Genetica Molecular,Depto. de Biologia, Bloco 906, Campus do Pici, Universidade Federal do Ceara, Av. Humberto Monte s/n, CEP 60.455-970, Fortaleza, Ceara,Brazil. ² Laboratorio de Genetica Molecular,Depto. de Biologia, Bloco 906, Campus do Pici, Universidade Federal do Ceara, Av. Humberto Monte s/n, CEP 60.455-970, Fortaleza, Ceara,Brazil. ³ Laboratorio de Genetica Molecular,Depto. de Biologia, Bloco 906, Campus do Pici, Universidade Federal do Ceara, Av. Humberto Monte s/n, CEP 60.455-970, Fortaleza, Ceara,Brazil. ⁴ Laboratorio de Genetica Molecular,Depto. de Biologia, Bloco 906, Campus do Pici, Universidade Federal do Ceara, Av. Humberto Monte s/n, CEP 60.455-970, Fortaleza, Ceara,Brazil. ⁵ Laboratorio de Genetica Molecular,Depto. de Biologia, Bloco 906, Campus do Pici, Universidade Federal do Ceara, Av. Humberto Monte s/n, CEP 60.455-970, Fortaleza, Ceara,Brazil. ⁶ Laboratorio de Genetica Molecular,Depto. de Biologia, Bloco 906, Campus do Pici, Universidade Federal do Ceara, Av. Humberto Monte s/n, CEP 60.455-970, Fortaleza, Ceara,Brazil.
Source: Protein and Peptide Letters, Volume 13, Issue 10, Oct 2006, p. 1045 - 1049
DOI: https://doi.org/10.2174/092986606778777434
- Available online: 01 Oct 2006

Abstract

Tobacco plants were transformed with gene constructs encoding prepro-ConBr (Canavalia brasiliensis lectin). Transgenic plants confirmed by PCR expressed the recombinant protein as revealed by Western blot. However, the apparent molecular mass of the recombinant polypeptide (ca. 34 kDa) was higher than the native lectin (about 30 kDa), showing that further proteolytic processing of pro-ConBr was not detected.

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2006-10-01

2025-05-31

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Article Type: Research Article

Keyword(s): Canavalia brasiliensis; heterelogous expression; lectin; precursor; processing; recombinant

Expression of the Canavalia brasiliensis Lectin (ConBr) in Tobacco Plants

Abstract

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