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2000
Volume 13, Issue 8
  • ISSN: 0929-8665
  • E-ISSN: 1875-5305

Abstract

A new, simple and sensitive method for the quantitative analysis of cytochrome C (Cyt C) based on the reduction wave of guanidine modified Co(II)-Cyt C complex at about -1.74 V (vs. SCE) by single sweep polarography in the solution containing 8'10-6 mol L-1 CoCl2, 0.04 mol L-1 guanidine hydrochloride, 0.2 mol L-1 NaOH and 0.5% Na2SO3. The peak height is linearly proportional to the concentration of Cyt C in the range of 0.005∼1.500 mg L-1 (correlation coefficient 0.999). Common amino acids, saccharide, organic acid and metal ions of appropriate concentrations have no interference on the Cyt C determination. The released Cyt C in the process of mitochondrial permeability transition of Hong-Lian cytoplasmic male sterile line of rice has been measured by the method, and the result is satisfactory.

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/content/journals/ppl/10.2174/092986606777841235
2006-08-01
2025-06-11
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  • Article Type:
    Research Article
Keyword(s): adsorptive polarographic wave; Co(II); Cytochrome C; guanidine; mitochondria; rice
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