Quantitative Analysis of Cytochrome C Released from Rice Mitochondria Using the Adsorptive Polarographic Wave of Guanidine Modified Co(II)- cytochrome C Complex

A new, simple and sensitive method for the quantitative analysis of cytochrome C (Cyt C) based on the reduction wave of guanidine modified Co(II)-Cyt C complex at about -1.74 V (vs. SCE) by single sweep polarography in the solution containing 8'10-6 mol L-1 CoCl2, 0.04 mol L-1 guanidine hydrochloride, 0.2 mol L-1 NaOH and 0.5% Na2SO3. The peak height is linearly proportional to the concentration of Cyt C in the range of 0.005∼1.500 mg L-1 (correlation coefficient 0.999). Common amino acids, saccharide, organic acid and metal ions of appropriate concentrations have no interference on the Cyt C determination. The released Cyt C in the process of mitochondrial permeability transition of Hong-Lian cytoplasmic male sterile line of rice has been measured by the method, and the result is satisfactory.