Production of Minichaperone (sht GroEL191-345) and Its Function in the Refolding of Recombinant Human Interferon Gamma

Yi-Xin Guan; Zheng-Zheng Fei; Man Luo; Shan-Jing Yao; Man-Gi Cho

doi:10.2174/0929866053405995

ISSN: 0929-8665
E-ISSN: 1875-5305

Production of Minichaperone (sht GroEL191-345) and Its Function in the Refolding of Recombinant Human Interferon Gamma
Authors: Yi-Xin Guan¹, Zheng-Zheng Fei², Man Luo³, Shan-Jing Yao⁴ and Man-Gi Cho⁵
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¹ Department of Chemical and Biochemical Engineering, Zhejiang University, Hangzhou 310027 P. R. China. ² Department of Chemical and Biochemical Engineering, Zhejiang University, Hangzhou 310027 P. R. China. ³ Department of Chemical and Biochemical Engineering, Zhejiang University, Hangzhou 310027 P. R. China. ⁴ Department of Chemical and Biochemical Engineering, Zhejiang University, Hangzhou 310027 P. R. China. ⁵ Department of Chemical and Biochemical Engineering, Zhejiang University, Hangzhou 310027 P. R. China.
Source: Protein and Peptide Letters, Volume 12, Issue 1, Jan 2005, p. 85 - 88
DOI: https://doi.org/10.2174/0929866053405995
- Available online: 01 Jan 2005

Abstract

The recombinant minichaperone sht GroEL191-345 was cultivated in a 3.7 L stirred bioreactor with the high yield of 216.2 mg / L broth. In the refolding of recombinant human interferon gamma (rhuIFN-γ) inclusion bodies, more than 2-3 fold enhancement in protein mass recovery and total activity were observed in the presence of free or immobilized minichaperone to the refolding buffer.

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2005-01-01

2025-05-22

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Article Type: Review Article

Keyword(s): cultivation; minichaperone; purification; recombinant human interferon gamma; refolding; sht groel191-345

Production of Minichaperone (sht GroEL191-345) and Its Function in the Refolding of Recombinant Human Interferon Gamma

Abstract

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