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- ISSN: 0929-8665
- E-ISSN: 1875-5305
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Site-Directed Mutagenesis and Preliminary X-Ray Crystallographic Studies of the Tabtoxin Resistance Protein
- Source: Protein and Peptide Letters, Volume 10, Issue 3, Jun 2003, p. 255 - 263
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- 01 Jun 2003
Abstract
Tabtoxin resistance protein (TTR) is an enzyme that catalyzes the acetylation of tabtoxin rendering tabtoxin-producing pathogens tolerant to their own phytotoxins. According to the structure based detoxification mechanism of TTR, three site-directed mutants Y141F, D130N and Y141F-D130N were constructed and overexpressed in E. coli. The products were then purified and their properties were analyzed by CD and DLS. Crystallization trials of two mutants Y141F andY141F-D130N were preformed.
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