Proliferative Activity Of Neokyotorphinrelated Hemoglobin Fragments In Cell Cultures

α-Hemoglobin fragments α-(133-141), α-(134-141), α-(135-141), α-(137-141), α-(134-140), α- (133-138), α-(134-140) and α-(137-138) stimulate L929 tumor cell proliferation, α-(134-141) being the most active. α-(134-141) stimulates proliferation of M3 melanoma cells, murine embryonic fibroblasts, primary cultures of red bone marrow and spleen cells. In L929 cells the effect of α-(134-141) is cell density independent; in M3 cells α-(137-141) and α-(134-141) are most active at density 10,000 cells / well (96 well plate) independently on FBS content.