Skip to content
2000
  1. Home
  2. A-Z Publications
  3. Protein and Peptide Letters
  4. Volume 31, Issue 11
  5. Article
Volume 31, Issue 11
  • ISSN: 0929-8665
  • E-ISSN: 1875-5305

Abstract

Background

Aloperine (ALO) is an important active ingredient in the traditional Chinese medicinal plant L and has a significant autophagy-stimulating effect. The effect of ALO on cytotoxicity caused by UVB radiation in skin fibroblasts and the potential mechanism remains unclear.

Objective

The present study aimed to assess the effect of ALO on UVB-induced damage in skin fibroblasts and investigate its possible mechanism.

Methods

Cell viability, cytotoxicity, caspase-Glo 3/7 activity, apoptosis, and protein expression were measured in UVB-treated skin fibroblasts in the presence or absence of ALO. Autophagy inhibitors (chloroquine and bafilomycin A1) and TFE3 siRNA transfection were used to elucidate the potential mechanisms further.

Results

These data demonstrate that ALO attenuated cell viability inhibition, apoptosis, cytotoxicity, and alterations in autophagy-related proteins caused by UVB exposure in skin fibroblasts. ALO stimulates autophagy activation and TFE3 nuclear localization in UVB-treated skin fibroblasts. Furthermore, treatment with autophagy inhibitors and TFE3 siRNA reversed the effects of ALO on UVB-treated skin fibroblasts.

Conclusion

These results suggest that ALO protects skin fibroblasts against UVB-induced cytotoxicity by stimulating TFE3/Beclin-1-mediated autophagy.

© 2024 Bentham Science Publishers
Loading

Article metrics loading...

/content/journals/ppl/10.2174/0109298665335370241017055831
2024-11-15
2025-01-31

  • From This Site

    /content/journals/ppl/10.2174/0109298665335370241017055831
    dcterms_title,dcterms_subject,pub_keyword
    -contentType:Contributor -contentType:Concept -contentType:Institution
    10
    5
Loading full text...

Full text loading...

References

  1. SajeedaA. BhatA.M. GorkeS. WaniI.A. SidiquiA. AhmedZ. SheikhT.A. Naringenin, A flavanone constituent from Sea buckthorn pulp extract, prevents ultraviolet (UV)-B radiation-induced skin damage via alleviation of impaired mitochondrial dynamics mediated inflammation in human dermal fibroblasts and Balb/c mice models.J. Photochem. Photobiol. B2024256, 11294410.1016/j.jphotobiol.2024.11294438796981
     [Google Scholar]
  2. TangX. YangT. YuD. XiongH. ZhangS. Current insights and future perspectives of ultraviolet radiation (UV) exposure: Friends and foes to the skin and beyond the skin.Environ. Int.2024185, 10853510.1016/j.envint.2024.10853538428192
     [Google Scholar]
  3. FurukawaJ.Y. MartinezR.M. JácomeM.A.L. GómezC.T.S. ContrerasP.V.J. RosadoC. VelascoM.V.R. BabyA.R. Skin impacts from exposure to ultraviolet, visible, infrared, and artificial lights – A review.J. Cosmet. Laser Ther.2021231-21710.1080/14764172.2021.195076734669525
     [Google Scholar]
  4. MaJ. TengY. HuangY. TaoX. FanY. Autophagy plays an essential role in ultraviolet radiation-driven skin photoaging.Front. Pharmacol.202213, 86433110.3389/fphar.2022.86433136278173
     [Google Scholar]
  5. WangR. DengX. GaoQ. WuX. HanL. GaoX. ZhaoS. ChenW. ZhouR. LiZ. BaiC. Sophora alopecuroides L.: An ethnopharmacological, phytochemical, and pharmacological review.J. Ethnopharmacol.2020248, 11217210.1016/j.jep.2019.11217231442619
     [Google Scholar]
  6. YuH.I. ShenH.C. ChenS.H. LimY.P. ChuangH.H. TaiT.S. KungF.P. LuC.H. HouC.Y. LeeY.R. Autophagy modulation in human thyroid cancer cells following aloperine treatment.Int. J. Mol. Sci.20192021531510.3390/ijms2021531531731481
     [Google Scholar]
  7. TangT. LiangH. WeiW. HanY. CaoL. CongZ. LuoS. WangH. ZhouM.L. Aloperine targets lysosomes to inhibit late autophagy and induces cell death through apoptosis and paraptosis in glioblastoma.Mol. Biomed.2023414210.1186/s43556‑023‑00155‑x37975957
     [Google Scholar]
  8. YuanX.Y. MaH.M. LiR.Z. WangR.Y. LiuW. GuoJ.Y. Topical application of aloperine improves 2,4-dinitro- fluorobenzene-induced atopic dermatitis-like skin lesions in NC/Nga mice.Eur. J. Pharmacol.20116582-326326910.1016/j.ejphar.2011.02.01321371468
     [Google Scholar]
  9. YuanX.Y. LiuW. ZhangP. WangR.Y. GuoJ.Y. Effects and mechanisms of aloperine on 2, 4-dinitro- fluorobenzene-induced allergic contact dermatitis in BALB/c mice.Eur. J. Pharmacol.20106291-314715210.1016/j.ejphar.2009.12.00720006963
     [Google Scholar]
  10. KlapanK. SimonD. KaraulovA. GomzikovaM. RizvanovA. YousefiS. SimonH.U. Autophagy and skin diseases.Front. Pharmacol.202213, 84475610.3389/fphar.2022.84475635370701
     [Google Scholar]
  11. ZhangL. YuG. YuQ. WangL. WuL. TaoZ. DingJ. LinD. Baicalin promotes random-pattern skin flap survival by inducing autophagy via AMPK-regulated TFEB nuclear transcription.Phytother. Res.20233793926393810.1002/ptr.784937291961
     [Google Scholar]
  12. UllahA. RazzaqA. AlfaifiM.Y. ElbehairiS.E.I. MenaaF. UllahN. ShehzadiS. NawazT. IqbalH. Sanguinarine attenuates lung cancer progression via oxidative stress-induced cell apoptosis.Curr. Mol. Pharmacol.202417, e1876142926938310.2174/011876142926938323111906223338389415
     [Google Scholar]
  13. UllahA. AzizT. UllahN. NawazT. Molecular mechanisms of sanguinarine in cancer prevention and treatment.Anticancer. Agents Med. Chem.202323776577810.2174/187152062266622083112432136045531
     [Google Scholar]
  14. MizushimaN. KomatsuM. Autophagy: Renovation of cells and tissues.Cell2011147472874110.1016/j.cell.2011.10.02622078875
     [Google Scholar]
  15. LiY. HaoD. WeiD. XiaoY. LiuL. LiX. WangL. GanY. YanW. KeB. JiangX. Photoprotective effects of cannabidiol against ultraviolet-b-induced dna damage and autophagy in human keratinocyte cells and mouse skin tissue.Molecules20222719674010.3390/molecules2719674036235276
     [Google Scholar]
  16. YeH. LiF. ShenY. WuX. ZhaoL. ZhangH. YangJ. ShuiX. Rosuvastatin promotes survival of random skin flaps through AMPK-mTOR pathway-induced autophagy.Int. Immunopharmacol.2023118, 11005910.1016/j.intimp.2023.11005937001384
     [Google Scholar]
  17. ShiM. ZongX. ChenL. GuoX. DingX. MiR-506-3p regulates autophagy and proliferation in post-burn skin fibroblasts through post-transcriptionally suppressing Beclin-1 expression.In Vitro Cell. Dev. Biol. Anim.202056752253210.1007/s11626‑020‑00472‑332754856
     [Google Scholar]
  18. NguyenT.N. LazarouM. A unifying model for the role of the ATG8 system in autophagy.J. Cell Sci.202213511, jcs25899710.1242/jcs.25899735665638
     [Google Scholar]
  19. TangS.C. KoJ.L. LuC.T. LeongP.Y. OuC.C. HsuC.T. HsiaoY.P. Chloroquine alleviates the heat-induced to injure via autophagy and apoptosis mechanisms in skin cell and mouse models.PLoS One2022178e027279710.1371/journal.pone.027279736044415
     [Google Scholar]
  20. WangQ. YouT. FanH. WangY. ChuT. PonczM. ZhuL. Rapamycin and bafilomycin A1 alter autophagy and megakaryopoiesis.Platelets2017281828910.1080/09537104.2016.120443627534900
     [Google Scholar]
  21. DengY. XuJ. ZhangX. YangJ. ZhangD. HuangJ. LvP. ShenW. YangY. Berberine attenuates autophagy in adipocytes by targeting BECN1.Autophagy201410101776178610.4161/auto.2974625126729
     [Google Scholar]
  22. XuH.D. QinZ.H. Beclin 1, Bcl-2 and autophagy.Adv. Exp. Med. Biol.2019120610912610.1007/978‑981‑15‑0602‑4_531776982
     [Google Scholar]
  23. LaiY. YangN. ShiD. MaX. HuangY. LuJ. ZhangX. ZhouH. GaoW. MaoC. WangL. Puerarin enhances TFEB-mediated autophagy and attenuates ROS-induced pyroptosis after ischemic injury of random-pattern skin flaps.Eur. J. Pharmacol.2024974, 17662110.1016/j.ejphar.2024.17662138679118
     [Google Scholar]
  24. DangT.T. BackS.H. Translation inhibitors activate autophagy master regulators TFEB and TFE3.Int. J. Mol. Sci.202122211208310.3390/ijms22211208334769510
     [Google Scholar]
  25. LiJ. ChenH. LouJ. BaoG. WuC. LouZ. WangX. DingJ. LiZ. XiaoJ. XuH. GaoW. ZhouK. Exenatide improves random-pattern skin flap survival via TFE3 mediated autophagy augment.J. Cell. Physiol.202123653641365910.1002/jcp.3010233044023
     [Google Scholar]
  26. YeB. WangQ. HuH. ShenY. FanC. ChenP. MaY. WuH. XiangM. Restoring autophagic flux attenuates cochlear spiral ganglion neuron degeneration by promoting TFEB nuclear translocation via inhibiting MTOR.Autophagy2019156998101610.1080/15548627.2019.156992630706760
     [Google Scholar]
  27. PanH.Y. AlamriA.H. ValapalaM. Nutrient deprivation and lysosomal stress induce activation of TFEB in retinal pigment epithelial cells.Cell. Mol. Biol. Lett.20192413310.1186/s11658‑019‑0159‑831160892
     [Google Scholar]
/content/journals/ppl/10.2174/0109298665335370241017055831
Loading
Aloperine Attenuates UVB-induced Damage in Skin Fibroblasts via Activating TFE3/Beclin-1-Mediated Autophagy
PPL 31, 884 (2024); https://doi.org/10.2174/0109298665335370241017055831
/content/journals/ppl/10.2174/0109298665335370241017055831
/content/journals/ppl/10.2174/0109298665335370241017055831
Loading

Data & Media loading...


  • Article Type:     Research Article
Keyword(s): Aloperine; autophagy; Beclin-1; skin fibroblasts; TFE3; UVB-induced damage

Most Cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error
Please enter a valid_number test