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Volume 5, Issue 1
  • ISSN: 0250-6882
  • E-ISSN: 0250-6882
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Abstract

Introduction

Adsorption and mixed agglutination reactions detect A, B, and H blood group antigens in the cells.

The study aimed to investigate the adsorbing abilities of group-specific polyclonal antibodies using erythrocytes and leukocytes to improve the quality of blood typing.

Material and Methods

The adsorption was performed with group B erythrocytes and leukocytes using the usual heated citrated anti-B plasma at 4°C and 37°C. A mixed agglutination reaction revealed group B antigens on leukocytes and erythrocytes.

Results

The highest adsorption of antibodies from usual plasma on erythrocytes was observed at 4°C at physiological conditions. Weaker adsorption was determined at pH 8.0 compared to pH 6.0 (the decrease of agglutination strength from 3+ to 2+ and 1+ accordingly).

The adsorption of polyclonal antibodies on erythrocytes at 37°C was the highest at pH 6.0 and 8.0 compared to physiological conditions (the decrease of agglutination strength from 3+ to 1+). In contrast, adsorption at 4°C appeared to be stronger at pH 6.0.

The adsorption of IgG antibodies on erythrocytes at 37°C was higher at pH 8.0.

Complete adsorption of IgG antibodies was observed on group B erythrocytes and leukocytes at 37°C and 4°C.

Conclusions

Adsorption of polyclonal group-specific antibodies on erythrocytes was the highest at 4°C, and adsorption of IgG antibodies was strong at 4°C and 37°C. Adsorption of leukocytes was revealed only with the use of the heated serum. IgG group-specific antibodies from the heated plasma demonstrated higher adsorbing abilities on leukocytes than IgM antibodies from plasma.

© 2024 The Author(s). Published by Bentham Science Publisher.
This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: https://creativecommons.org/licenses/by/4.0/legalcode. This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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2024-01-01
2025-01-19

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/content/journals/nemj/10.2174/0102506882279058240106123702
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The Differences of Blood Group Typing using Erythrocytes and Leukocytes
NEW Emirates Medical J 5, e02506882279058 (2024); https://doi.org/10.2174/0102506882279058240106123702
/content/journals/nemj/10.2174/0102506882279058240106123702
/content/journals/nemj/10.2174/0102506882279058240106123702
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  • Article Type:     Research Article
Keyword(s): Adsorption; Agglutination; Erythrocytes; Leukocytes; Plasma; Transplantation

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