Current Pharmaceutical Analysis - Volume 18, Issue 10, 2022

Background: The pharmaceutical industry is in constant development, aiming to increase its portfolio, optimizing time, product quality and efficacy along with patient safety. The main goal of developing and validating an analytical method is to achieve a balance between costs and risks within the wide array of technical possibilities in order to assure that the method is capable of meeting its expectations, ensuring effective qual Read More

Background: Perampanel (PER) is a third-generation antiepileptic drug (AED). Several methods have been developed for the quantification of perampanel in plasma. The pharmacokinetic characteristics of perampanel in healthy Chinese ubjects have not been comprehensively reported. Objective: A simple, fast and sensitive LC-MS/MS method was established and validated for the quantification of perampanel in human pl Read More

Background: Polymyxin E (PME), a complex of cationic cyclic lipodecapeptides, is used to treat multidrug-resistant gram-negative bacterial infections. Besides the main components PME1 and PME2, polymyxin containing unsaturated fatty acyl (FA) group with lower contents can hardly determine the structure without chromatographic preparations and NMR. Introduction: The peptide sequences of PME components have Read More

Background: To explore the pharmacokinetic data of Astragali Radix, a simple, one-step deproteinization procedure was followed to prepare plasma samples, and separation was achieved on an InfinityLab Poroshell 120 column (3.0 mm × 50 mm, 1.8 μm) with a gradient mobile phase consisting of solution A (water containing 0.1% formic acid) and solution B (methanol) at a flow rate of 0.3 mL/min. Aims: In this study, a rapid, sen Read More

Background: Non-steroidal anti-inflammatory drugs (NSAIDs) are widely used worldwide for their analgesic, anti-inflammatory, and antipyretic properties. Objective: The article highlights the development of zwitterionic hydrophilic interaction liquid chromatography (ZIC-HILIC) methods to quantify three NSAIDs as active ingredients in pharmaceutical formulations simultaneously. Methods: For NSAIDs analysis, two homemad Read More

Background: Recombinant activated human coagulation factor VIIa (rFVIIa), a vitamin K-dependent serine protease, was originally developed by Novo Nordisk® for the treatment of patients with FVII deficiency as well as patients with hemophilia A and B with inhibitors against FVIII or FIX. The gene for human FVII is cloned and expressed in baby hamster kidney (BHK) cells, and the protein is secreted into the culture media, whi Read More

Background: Mixtures of Fluorinated Quinolones and Nitroimidazole antibacterial drugs take a significant place in the treatment of different inflammatory diseases. The necessity to develop analytical techniques for quality control is inextricably related to the introduction of new mixed dose forms into clinical practice. Objective: The objective of our study is to develop HPLC methods for the analysis of Fluorinated Quinol Read More

Background: Fisetin (FIS) is a bioactive flavonoid found in various plants, reported for many pharmacological activities, and presently marketed as a nutraceutical. To overcome less water solubility and bioavailability issues, FIS cubosomal nanoformulation has been prepared and characterized. Objective: To estimate FIS in prepared novel cubosomes, an RP-HPLC analytical method development with the most sensitivity, economi Read More