Current Pharmaceutical Analysis - Volume 14, Issue 4, 2018

Background: 7, 8-dihydroxyflavone (7, 8-DHF) has been discovered to be a TrkB receptorspecific agonist and demonstrated remarkable therapeutic efficacy in vivo. These promising findings prompted the current pharmacokinetic study, in which we first established a practical LC-MS quantitative method for 7, 8-DHF then applied it in a pilot pharmacokinetic study in rats. Methods: A simple HPLC-MS system was employed for the d Read More

Background: Fraxetin is mainly an active ingredient of Fraxini Cortex which is an important and traditional Chinese medicine for clinical applications, has heat-clearing, dispelling dampness, antitussive and anti-asthmatic effects. Fraxetin has a variety of pharmacological activities. Method: The chromatographic separation was carried out on a Zorbax Eclipse XDB C18 column. The mobile phase consisted of acetonitrile (0.1 % for Read More

Objective: A simple and rapid method for the measurement of paracetamol in oral pharmaceutical preparations by a commercial glucometer was proposed. Methods: The established method was successfully applied to the determination of paracetamol in oral solid preparations, and exhibited the excellent capability of resisting interference from co-existing excipients. The commercial disposable test strips of hand-held glucome Read More

Background: Clorsulon is a drug substance in other words Active Pharmaceutical Ingredient (API). It is listed in the United States Pharmacopoeia (USP). The related substances analysis as per USP is by Thin Layer Chromatography (TLC). It has been extremely difficult for the analyst to take decision on quality as the test was not able to reproduce the result and hence the purity by HPLC was planned. It was observed from the Read More

Background: Tolterodine was quantitatively determined using variable analytical methods including; spectrophotometric and chromatographic methods. Spectrophotometric methods are usually fast, easy to handle, cost effective and very reliable for the routine assay during in process quality control procedures. Eosin Y has been used to determine several basic compounds through the formation of colored complexes. I Read More

Background: Protodioscin, a furostanolic saponin type phytocompound, is claimed to have aphrodisiac and anticancer activities. Determination of protodioscin is challenging problem since it lacks chromophoric or florophoric groups. Pharmacokinetic data after administration of protodioscinrich extract prepared from Trigonella foenum-graecum in rabbit was not yet reported. A selective and sensitive LC–MS/MS method with solid Read More

Purpose: To develop two PVC membrane sensors for determination of ciprofloxacin (CIP). Method: Beta - or γ-cyclodextrin was investigated as electroactive material for the formation of sensor 1 and 2 in the presence of potassium tetrakis (4-chlorophenyl) borate (KTpClPB) as ion additive. Results: The investigated sensors appeared fast, accurate, sensitive and showed near-Nernstian response (50 mV/ decade) with a calibr Read More

Background: Penthorum chinense Pursh has a long history as a traditional medicine for the treatment of liver diseases in China. Despite its common use, investigations on the hepatoprotective components of P. chinense have not been carried out clearly. Experiment: Four extraction methods related to reflux, cold-soak, microwave and ultrasonic extraction were compared for optimum condition, combined with in vitro antioxid Read More

Background: In this work, we developed and validated a simple, rapid and accurate reverse- HPLC for the multi-determination of enrofloxacin (EFX), ciprofloxacin (CFX), and oxytetracycline (OXY) in raw materials and veterinary pharmaceutical formulations. Method: Optimum chromatographic separations among these three analytes have been achieved within 3 min employing an Agilent Poroshell 120 EC-C18 Read More

Background: Daptomycin (DPT) is the first lipopeptide antibiotic available for commercialization, approved by FDA in 2003 and not included in any official compendia. Introduction: A simple capillary zone electrophoresis method (CZE) to assay DPT injection was developed according to international guidelines. Methods: The method employed a 15 mmol L-1 pH 8.0 phosphate buffer and acetonitrile (85:15) as background Read More

Objective: To devise a rapid, simple, selective and sensitive High Performance Liquid Chromatography (HPLC) method for simultaneous estimation of hypertension drug Valsartan and diabetic drug Gliclazide in bulk and dosage forms. Methods: Valsartan and Gliclazide were measured using the HPLC method with UV detector at 234 nm wave length. Drug analytes were separated on 5μm inertsil C18 column (4.6x250mmx5μm) u Read More

Background: A mixture of aspirin and dipyridamole has been proved to significantly reduce the stroke recurrence. As the use of mixture of aspirin and dipyridamole is increased, the accurate concentration of each component should be determined to avoid side effects and to improve the treatment process in pharmaceutical preparation. Objective: The goal of the present study is the simultaneous determination of aspirin Read More