Current Pharmaceutical Analysis - Volume 14, Issue 5, 2018

Background: Mesenchymal Stem Cells (MSCs) are multipotent stem cells with therapeutic potential in advanced therapies and regenerative medicine. Currently, MSCs are being widely studied as cellular medicine. In the design and development of medicines with cells, the study of viability is an important quality control requirement. Objective: Description of the main analytical techniques to study the MSCs viability a Read More

Background: Cabergoline, used for treating hyperprolactinemia, is hard to detect and determine in biological samples due to its dosage form. Materials and Methods: The trend hollow fiber mediating technique was used for preconcentration and extraction of cabergoline using the solvent bar and HPLC-UV. The parameter affecting the efficiency of this technique was optimized. The pH gradient was adjusted in accordance Read More

Background: Acidic polysaccharides usually possess favorable bioactivities, and some of them are applied in medicines and dietary supplements together. However, it is a challenge to quantify these polysaccharides simultaneously. Methods: A method to Quantify Hyaluronic Acid (HA), Chondroitin Sulfate (CS) and a nonglycosaminoglycan acidic polysaccharide named AGSP was established by determining the contents o Read More

Introduction: The acute treatment of migraine headaches is one of the major fields in which rizatriptan is used. Materials and Methods: To determine rizatriptan in biological fluids, many analytical approaches have been reported based on Liquid Chromatography/ Tandem Mass Spectrometry (LC-MS/MS), High- Performance Liquid Chromatography (HPLC), Reversed Phase Liquid Chromatography with Diode Array Read More

Background: Two sensitive and accurate methods have been developed for the determination of ceftriaxone sodium and sulbactam sodium in presence of their acidic, alkaline, oxidative, neutral and photolytic degradation products by chromatography including TLC and HPLC methods, as stability- indicating techniques. Methods: The first one is RP-HPLC method based on isocratic elution of the two drugs with their degradat Read More

Background: This study investigates fatty acids and sterol contents of some seeds used in Asian nutrition culture to prepare functional beverages, foods or fatty acids and sterols source. Objective: Current study presents an overview about some seeds as a valuable source for fatty acids and sterols extraction. Methods: Gas chromatography-mass spectrometry was employed to quantify fatty acids and sterol contents and com Read More

Background: Sitagliptin (SITA), is an oral anti-diabetic drug of dipeptidyl peptidase-4 (DPP-4) inhibitor class used as a monotherapy or in association with metformin to treat type II Diabetes Mellitus. Methods: Two new, sensitive and precise spectrofluorimetric methods have been developed for the determination of SITA in tablets and spiked human urine samples. SITA has a native fluorescence that was enhanced by 2 folds after Read More

Background: Many methods have been reported for the determination of ceftazidime such as liquid chromatography, spectrophotometry and electrochemical methods. Among these methods, electrochemical methods have their unique advantages such as rapidity, low cost and simplicity. Methods: In this work, electrochemical technique was used for the determination of ceftazidime using a carbon nanotube modified elect Read More

Background: Traditional Chinese medicine has been widely used in China for a long time. As a new type of formulation, Traditional Chinese Medicine Injection (TCMI) is diffusely used clinically as a promising therapy for a variety of diseases in primary health care institutions of China, which has many advantages, such as functional reliability and speed of action. In particular, TCMI is very suitable for rescue in an emergency. Obje Read More

Background: The objective of present research work was to develop and validate rapid UHPLC method with short run time for the detection and quantification of recombinant human insulin content in microsphere formulations. Validation emphasizes mainly on studying the effects of the flow rate, wavelengths, column temperature, pH and organic content of the mobile phase and of presence of other additives on the method' Read More

Background: Estradiol valerate tablet is used for ovarian dysfunction, menopause, menopausal syndrome, breast and prostate cancer. Introduction: HPLC method coupled with photodiode-array detection has been used to develop a simple and sensitive reversed-phase method for quantitative determination of all possible related impurities in Estradiol valerate tablet. Methods: Zorbax eclipse XDB C8(150mmx4.6mm,5μm) colum Read More

Background: Cetuximab (CET) is a monoclonal antibody that inhibits Epidermal Growth Factor Receptor (EGFR) used for immunotherapy of different types of cancer. Additionally, CET used in combination therapy, potentiates the effects of chemotherapy and radiation therapy in eradicating well-established tumors. Recently, a combination of CET and newly developed chemotherapeutic candidate drugs are being investigated for Read More