Current Pharmaceutical Analysis - Volume 14, Issue 1, 2018

Objective: To establish a particular, sensitive and accurate High Performance Liquid Chromatography (HPLC) technique for the specific and effective analysis of 5-Fluorouracil (5-FU) in mobile phase and in plasma. Method: Analytical methods for 5-FU in mobile phase and plasma were developed. An assay consisted of isocratic elution of 5-FU in prepacked column discovery R-HS C-18, 5micrometer, Catalog # 568523-U, column di Read More

Background: Polymorphism in drugs is a common phenomenon which can lead to compromised quality due to changes in their physicochemical properties and may impact therapeutic efficacy. Objective: The main objective of the present study is to investigate the polymorphic behaviour of Pioglitazone Hydrochloride (PGH) under different preparative conditions and to evaluate the solid forms. Method: The solid-state forms o Read More

Background: Sulfonamide antibiotics are the second most frequent cause of allergic drug reactions (after the β-lactams). Activity and side effects of sulfonamide may be attributed to their metabolites. To the best of our knowledge there is no in vitro metabolism study for sulfamerazine (SMR) available in the literature. The main objectives of this research are to develop an analytic method for screening, identifying and quantifyi Read More

Background: Vitamin has an important function in biochemical processes and plays an important role in the main process of the human body. Vitamin products are used to maintain health, restore vitality, control weight and help in disease prevention. To comply with regulatory requirements and to establish nutrient intake in some populations, the quality of vitamins in food and nutritional supplements needs to b Read More

Background: Fifteen pairs of geometric isomers of new sulfonamide derivatives, exhibiting antitumor activities were analyzed with the use of quantitative structure-retention relationships method. The set of studied compounds bears thiohydrazone moiety, which leads to in-solution formation of an equilibrium between cis and trans isomer. The semi-empirical level of in silico molecular modeling was performed for ca Read More

Objective: This study aims to establish an efficient, sensitive and specific analytical method (in vitro and in vivo) for syringic acid. Method: Syringic acid loaded liposome was prepared and characterized. In vitro and in vivo chromatographic conditions for the determination of syringic acid were investigated, with the application verified by pharmacokinetics and biodistribution studies. Results: The developed liposomal formulation Read More

Background: The combination of cyclophosphamide and doxorubicin is commonly used in the adjuvant treatment of breast cancer in women with a high risk of recurrent disease. It is essential to devise dosing strategies for such drugs in order to minimise their in-use toxicity and to maximise their efficacy. Data have shown that concentration directed rather than dose targeted therapy provides better clinical outcomes. Theref Read More

Background: Simultaneous determination of binary mixtures with simple and cost effective analysis is always of interest. Progressive advancement in chemometrics enables spectral resolution of drugs in the presence of their degradation products or impurities. Methods and Result: Three stability indicating chemometric methods are applied for the simultaneous determination of Dantrolene sodium (DNT) and Paracetamol Read More

Introduction: Chromatographic methods for determination of betamethasone or dexamethasone in their pharmaceutical preparations by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry were developed. Method: Analytes were eluted from Acquity BEH C18 (50 mm.1 mm, 1.7 μm) column using stepwise gradient with mobile phase consisted of acetonitrile and 0.1% (v/v) acetic acid in water. Quantitatio Read More

Background: The field of research in pharmacy is rapidly shifting towards nanocarrier based drug delivery systems. There is a need to develop accurate and specific analytical techniques for the analysis of the drugs in nanocarriers. The performance of such system will be based on how accurately these are analyzed during their development stage. Aim and Objective: The present study deals with the development, valida Read More