Current Pharmaceutical Analysis - Volume 14, Issue 6, 2018

Background: Analytical method development and validation remain the key prerequisites for the successful development of a product. Methods: An endeavour, therefore, was made for the development of an effortless, swift, sensitive and cost-effective method for estimation of galantamine hydrobromide using the established paradigms of Quality by Design (QbD). Results: Chromatographic separation was accomplished on a Read More

Background and Objectives: To establish a simple and reproducible High Performance Liquid Chromatography (HPLC) method for the determination of mizoribine in human plasma using glipizide as Internal Standard (IS). Methods: The mizoribine in plasma was precipitated with 10% perchloric acid and separated on a Phenomenex Luna NH2 (250 mm 4.6 mm, 5 μm) column with a mobile phase consisted of acetonitrile and 0.3 Read More

Background: Teneligliptin is anti diabetic drug acting as dipeptidyl peptidase-4 inhibitor. HPLC methods are reported for the estimation of teneligliptin but till date no HPTLC method has been reported. HPTLC method has advantage of short analysis time, requires less quantity of mobile phase, and accurate and comparable results can be obtained with that of HPLC. Objective: Objective of the present study is to develop s Read More

Background: Exhaled breath has recently been identified as an alternative matrix for the detection of drugs and chemical compounds. By keeping in mind that different chemical and biological compounds have already been determined in the exhaled air, the Exhaled Breath Condensate (EBC) was found to be a suitable sample for non-invasive assessment of the respiratory system. Thus, the aim of this work is determination of Read More

Background: Ganoderma lucidum is a well-known edible mushroom and traditional Chinese medicine, but there were no comprehensive reports on the extraction, analysis and activities test of Ganoderma lucidum Amino Acids (GLAA). Objective: The present study was aimed to optimize the extraction process of GLAA and analyze the amino acid composition of GLAA, combined with preliminary activity evaluation. Methods: T Read More

Background: Green electrochemical sensor (an in-situ plated bismuth film glassy carbon electrode) was used for the first time in combination with adsorptive striping voltammetry to elaborate the novel, simple, cheap and sensitive alternative analytical procedure for determination of rutin. Methods and Results: In this paper, the electrochemical procedure for the determination of rutin using a square-wave adsorptive strippi Read More

Background: Eremurus spectabilis BIEB. (Liliaceae) is an edible and medicinal plant in Turkey. Introduction: This study was designed to isolate and quantify isoorientin in leaves of E. spectabilis and exhibit its effect on SH-SY5Y neuroblastoma cell line. Methods: Purity and identification of isoorientin were evaluated by 1D-NMR, 2D-NMR and Q-TOF were isolated from E. spectabilis via column chromatography. An HPLC meth Read More

Background: Cuphea glutinosa is a medicinal species extensively distributed in South of Brazil, being known in the folk medicine as diuretic, anti-inflammatory, laxative and anti-hypertensive. In the present work, its majority chemical components present in infusions and macerations obtained from leaves and roots were investigated. The analytical responses were well worked using a UPLC-MS, whose detection was standardize Read More

Background: A new UHPLC method was developed and subsequently validated for determination of the anti-hypertensive combination Trandolapril (TAD) and verapamil hydrochloride (VRP) in bulk powder, dosage form (Tarka® 2 mg TAD/ 180 mg VRP), and spiked human plasma. Moreover, the method was utilized for studying the stability of the combination under hydrolytic and thermal conditions. Methods: Separation was achi Read More

Objective: A simple, sensitive, robust method for the estimation of zaltoprofen was developed and validated. The forced degradation study was carried out as per FDA guidelines. Methods: The method was developed by using HPLC Binary Gradient System (Model no.: HPLC 3000 Series) of Analytical Technologies Ltd. equipped with the UV-3000-M detector, P-3000-M Reciprocating (40MPa) Pump, Grace C18 (250mm x 4.6ID, Read More

Background: Most of the fat-soluble vitamins are not stable and decompose when exposed to light. Therefore, stability testing of the analytes in solutions and biological matrix is required during development and validation of analytical methods devoted to determination of these vitamins in biological fluids. Objective: Determination of the stability of lipophilic vitamins, including retinol, α-tocopherol, β- carotene and 25-hydrox Read More

Background: Synthesis of substituted N'-aryl-N'-adipic acid thiourea (1-7) was, carried out adipic acid chlorides with potassium thiocynate in situ produced isocyanate followed by reflux with substituted anilines in dry acetone till the completion of reaction. Methods: Structure elucidation of all the synthesized compounds was carried out by UV-Vis, FT-IR and 1HNMR analysis. All the synthesized compounds were evaluated for the Read More

Background: The purpose of this study is the development and validation of assay test for Dapoxetine Hydrochloride in the pharmaceutical dosage forms by HPLC. The assay method by HPLC was found to be linear in the concentration range of 25 to 150 μg/mL. The mobile phase was composed of methanol and buffer pH: 7.0 ammonium dihydrogen phosphate (80:20 v/v) at the flow rate of 1 mL/min using UV detection at 232 nm Read More

Background: Non-ionic X-ray contrast agents constitute a very important class of pharmaceutical compounds produced in large quantities. Iohexol is an important example of such compounds. Objective: Three simple and selective stability indicating spectrophotometric methods utilizing ratio spectra were proposed for the determination of the widely used X-ray contrast medium, iohexol in the presence of its acidic degradate Read More

Introduction: Curcumin possesses a wide range of biological properties, including antiinflammatory, anti-oxidant, anti-proliferative, and anti-microbial activities. Paraquat (PQ) is a highly toxic herbicide, and many cases of acute poisoning and death have been reported over the past decades in humans and animals. In this study, we developed a tissue (lung, liver, kidney, heart) metabolomics method by Gas Chromato Read More