Current Pharmaceutical Analysis - Volume 14, Issue 3, 2018

Background: Performance enhancement substances and methods other than exercise training and physical conditioning have become a major problem in athletic competitions. Over the last few years, there has been an increase in the number of these doping approaches used by some athletes, including pharmaceuticals, slightly modified endogenous compounds and also blood transfusions. In order to control and preve Read More

Background: A growing attention was paid on the antitumor activities of Pulsatilla chinensis (Bunge) Regel. For better pharmacological elucidation and further safety evaluation, we characterized tissue distribution of five active pulchinenosides for solubilization formulations of Pulsatilla chinensis saponins in a tumour-bearing mice model. Hypothesis/Purpose: The active ingredients level at the site of action tissues is the relevant m Read More

Background: Spironolactone, an aldosterone antagonist and a diuretic, is unstable in aqueous solution. The stability-indicating method has not been reported for use in the assessment of spironolactone stability in the developed oral liquids. Objective: To develop and validate a stability-indicating HPLC method for determination of spironolactone in parabens-preserved oral liquids formulated with and without hydroxypropyl-β-cyclo Read More

Background: Recombinant human interleukin-11 (rhIL-11) is a cytokine produced by recombinant DNA technology and used clinically to treat chemotherapy-induced thrombocytopenia. A Size Exclusion Liquid Chromatography (SE-LC) method was validated to assess the content/potency of the rhIL-11. Methods: A BioSep-SEC-S2000 column (300 mm 7.8 mm i.d.) maintained at 30°C was used. The mobile phase was compose Read More

Objective: The present study describes the development and validation of a simple highthroughput method for the fluorometric determination of besifloxacin HCl in 96-microwell plates. Methods: The proposed method is based on the reaction between besifloxacin HCl and fluorescamine in borate buffer solution of pH 8.5 giving a highly fluorescent derivative that was developed simultaneously and measured at 471 nm after Read More

Introduction: The majority of analytical methods used currently to quantify Vitamin B6 are time-consuming, costly, non-environment friendly and destructive with respect to the analyzed material. Objective: The objective of this study was to elaborate a novel method of vitamin B6 quantification in commercial pharmaceutical preparations using Near Infrared Spectroscopy (NIRS). Methods: Powdered tablets and cali Read More

Background: A simple, sensitive and precise spectrodensitometric method has been developed and validated for simultaneous determination of sofosbuvir, ribavirin and saxagliptin in their pure and pharmaceutical dosage forms. Methods: The method employed TLC plates precoated with silica gel G 60 F254 as the stationary phase. The mobile phase consisting of acetonitrile-water (80:20, v/v) was used to give compact ban Read More

Background: Propylthiouracil (PTU)-induced liver injury has been concerned in recent years and the mechanisms are largely unknown. Therefore, establishment of an analytical method for PTU detection in hepatic cells would contribute to the investigation of PTU hepatic injury. Objectives: In this study, a simple and rapid high performance liquid chromatography (HPLC) method for the intracellular determination of PTU concen Read More

Background: It is highly crucial to clarify the pharmacokinetic mechanism for the active multi-ingredients in Traditional Chinese Medicines so that suggestions could be provided in clinical. Introduction: A novel, rapid and sensitive Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry (UPLC–MS/MS) method was developed for the simultaneous determination of four compounds (tetrahydropalmatine, dans Read More

Background: An accurate HPLC-fluorescence method for the simultaneous determination of abiraterone acetate (prodrug) and abiraterone (drug) employed in the treatment of prostate cancer, in urine and serum samples of men treated with this prodrug has been proposed. Methods: The developed HPLC-FLD procedure permits the quantification of ABR and AA minimizing laborious and complicated sample preparation pro Read More

Background: The determination of NSAIDs (ketoprofen, diclofenac, and ibuprofen) in urine samples provides useful information for assessing their safety, therapeutic effect, and their mechanism of action. Urine samples are characterized by their complexity and low concentration of the target analytes, which make their direct analysis difficult. In this work, the potential of multi-walled carbon nanotubes (MWCNTs) as Solid Bar Mi Read More

Background: Levofloxacin (LVX) is one of the fluoroquinolone antibiotics that possess a wide spectrum of bactericidal activity against both Gram-positive and Gram-negative bacteria. Because of its side effects and its potential towards the development of antibiotic resistance, determination of this antibiotic is very important. New methods which have superior properties than the conventional methods are needed in the determi Read More

Background: In this paper, an isocratic reverse phase liquid chromatography method was developed and validated for the determination of curcumin and quinine in nanoformulation using a photodiode array detector. Methods: The optimum chromatographic condition with adequate resolution for curcumin (3.8 minutes, 426 nm) and quinine (8.9 minutes, 232 nm) was achieved when the separation was carried out usi Read More

Background: Carbohydrates are of great interest for the synthesis of novel ribonucleosides and C-Nucleosides which often show different pharmacological potential including antiinflammatory and antineoplastic characteristics. Introduction: In this research twelve aldopentose derivatives were examined and their chromatographic properties were used to describe their pharmacokinetic profiles. Methods: Thin la Read More

Introduction: Piroxicam (PRX) is a widely consumed anti-inflammatory drug, and the assays of its tablets and gels for quality control are mostly performed through High Performance Liquid Chromatography with Ultraviolet Detector (HPLC-UV). Methods: In this regard, an alternate approach, LaFeO3 nanoparticles-modified carbon paste electrode was prepared in order to carry out PRX analysis by using voltammetric approach Read More

Background: A simple, precise, selective and Reproducible Reversed Phase Ultra-High Performance Liquid Chromatography (RP-UHPLC) method has been developed and validated for the simultaneous determination of two plant constituents, Curcumin (Cur) and Thymoquinone (THQ) in lipid based self-nanoemulsifying formulation and commercial products (Turmeric 500mg capsules and black seed oil 500ml). Method: The chromato Read More

Background: Quercetin (Qur), a potent antioxidant flavonoid have been reported with wide applications in cardiovascular and cerebral ischemic diseases however Qur is prone to degradation in different stress conditions i.e. oxidation, alkaline and acidic medium, photodegradation under UV-light as well as thermal and enzymatic degradation by intestinal bacteria. Objective: The aim of current research is; to study Qur degrad Read More

Background: Finasteride is a medically important compound belonging to 4-azasteroids which are widely used in the treatment of benign prostatic hypertrophy. Therefore, there is a need to find a simple, cost effective and sensitive method for the determination of finasteride in commercially available tablet dosage form. Objective: To develop and validate a rapid and simple RP-TLC method combined with densitometry for the Read More

Introduction: Until date, the methods employed in the quantification of midazolam during sedation and in pre-anesthetic patients are somewhat complex and costly. Hence, the development of simple, practical, precise and reliable method becomes extremely important to measure plasma midazolam levels that could allow its monitoring during sedation, especially in patients who need higher doses of midazolam to achieve Read More

Background: Ethionamide (ETA) is widely used as one of the agents for the treatment of multidrug resistant tuberculosis. Although quality control is an important issue, a fast LC-UV method for the assay and impurity determination of ETA was lacking. So, the aim of this study was to develop such a method to evaluate drug products and follow up dissolution tests of ETA tablets. Methods: Chromatographic separation was achieved Read More