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Volume 2, Issue 1
  • ISSN: 2666-1217
  • E-ISSN: 2666-1225

Abstract

Cholera is a life-threatening secretory diarrheal disease caused by bacterium. On the contrary, local and specific use of cholera toxin (CT) at a low concentration can cause controlled fluid secretion. In the study, we explored the secretory action of CT in the intestine of rats with acute renal failure (ARF).

Closed intestinal loop experiments were performed in ARF rats treated with CT. Secreted fluid and serum were analyzed for various solutes and electrolytes. The presence of K+, Na+, Cl-, urea and creatinine were monitored. Histopathology analysis was carried out to evaluate the effect of CT in liver, kidney, and intestinal tissues.

A reduction in the absorption of water and electrolytes was observed over time and a secretory response started to appear within hours of CT treatment. The fluid secretory response with entrapped electrolytes was profound in ARF rats. Histopathological analysis of CT exposed tissues revealed that apart from the tissue damage produced by acute renal failure, no CT induced cellular changes occurred.

CT can be used as a secretagogue to induce fluid and electrolyte secretion in ARF rats. However, effective measures should be taken to avoid CT induced acidosis.

© 2022 Bentham Science Publishers
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2022-04-01
2025-03-02

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References

  1. GoergK.J. GrossM. NellG. RummelW. SchulzL. Comparative study of the effect of cholera toxin and sodium deoxycholate on the paracellular permeability and on net fluid and electrolyte transfer in the rat colon.Naunyn Schmiedebergs Arch. Pharmacol.19803121919710.1007/BF005025806771659
     [Google Scholar]
  2. KrejsG.J. BarkleyR.M. ReadN.W. FordtranJ.S. Intestinal secretion induced by vasoactive intestinal polypeptide. A comparison with cholera toxin in the canine jejunum in vivo.J. Clin. Invest.19786151337134510.1172/JCI109051659596
     [Google Scholar]
  3. PetersonJ.W. OchoaL.G. Role of prostaglandins and cAMP in the secretory effects of cholera toxin.Science1989245492085785910.1126/science.25496372549637
     [Google Scholar]
  4. StrombeckD.R. The production of intestinal fluid by cholera toxin in the rat.Proc. Soc. Exp. Biol. Med.1972140129730310.3181/00379727‑140‑364445033104
     [Google Scholar]
  5. NocerinoA. IafuscoM. GuandaliniS. Cholera toxin-induced small intestinal secretion has a secretory effect on the colon of the rat.Gastroenterology19951081343910.1016/0016‑5085(95)90005‑57806061
     [Google Scholar]
  6. LeitchG.J. IwertM.E. BurrowsW. Experimental cholera in the rabbit ligated ileal loop: toxin-induced water and ion movement.J. Infect. Dis.1966116330331210.1093/infdis/116.3.3035940329
     [Google Scholar]
  7. SawasvirojwongS. SrimanoteP. ChatsudthipongV. MuanprasatC. An adult mouse model of Vibrio cholerae-induced diarrhea for studying pathogenesis and potential therapy of cholera.PLoS Negl. Trop. Dis.201376e229310.1371/journal.pntd.000229323826402
     [Google Scholar]
  8. ZhangR-G. ScottD.L. WestbrookM.L. NanceS. SpanglerB.D. ShipleyG.G. WestbrookE.M. The three-dimensional crystal structure of cholera toxin.J. Mol. Biol.1995251456357310.1006/jmbi.1995.04567658473
     [Google Scholar]
  9. SpanglerB.D. Structure and function of cholera toxin and the related Escherichia coli heat-labile enterotoxin.Microbiol. Rev.199256462264710.1128/mr.56.4.622‑647.19921480112
     [Google Scholar]
  10. GoodmanB.E. PercyW.H. CFTR in cystic fibrosis and cholera: from membrane transport to clinical practice.Adv. Physiol. Educ.2005292758210.1152/advan.00035.200415905150
     [Google Scholar]
  11. ZhangW. FujiiN. NarenA.P. Recent advances and new perspectives in targeting CFTR for therapy of cystic fibrosis and enterotoxin-induced secretory diarrheas.Future Med. Chem.20124332934510.4155/fmc.12.122393940
     [Google Scholar]
  12. ThiagarajahJ.R. DonowitzM. VerkmanA.S. Secretory diarrhoea: Mechanisms and emerging therapies.Nat. Rev. Gastroenterol. Hepatol.201512844645710.1038/nrgastro.2015.11126122478
     [Google Scholar]
  13. KumarP. ThomasS. Classical ctxB gene in Vibrio cholerae O1 and O56 serogroups from Kerala, South India.J. Med. Microbiol.201160Pt 455956010.1099/jmm.0.024752‑021212148
     [Google Scholar]
  14. WuJ. PanX. FuH. ZhengY. DaiY. YinY. ChenQ. HaoQ. BaoD. HouD. Effect of curcumin on glycerol-induced acute kidney injury in rats.Sci. Rep.2017711011410.1038/s41598‑017‑10693‑428860665
     [Google Scholar]
  15. PetritschW. EhererA.J. Holzer-PetscheU. HinterleitnerT. BeublerE. KrejsG.J. Effect of cholera toxin on the human jejunum.Gut19923391174117810.1136/gut.33.9.11741427368
     [Google Scholar]
  16. VicarioM. González-CastroA.M. MartínezC. LoboB. PigrauM. GuilarteM. de TorresI. MosqueraJ.L. ForteaM. Sevillano-AguileraC. Salvo-RomeroE. AlonsoC. Rodiño-JaneiroB.K. SöderholmJ.D. AzpirozF. SantosJ. Increased humoral immunity in the jejunum of diarrhoea-predominant irritable bowel syndrome associated with clinical manifestations.Gut20156491379138810.1136/gutjnl‑2013‑30623625209656
     [Google Scholar]
  17. ChengS.H. RichD.P. MarshallJ. GregoryR.J. WelshM.J. SmithA.E. Phosphorylation of the R domain by cAMP-dependent protein kinase regulates the CFTR chloride channel.Cell19916651027103610.1016/0092‑8674(91)90446‑61716180
     [Google Scholar]
  18. ThiagarajahJ.R. BroadbentT. HsiehE. VerkmanA.S. Prevention of toxin-induced intestinal ion and fluid secretion by a small- molecule CFTR inhibitor.Gastroenterology2004126251151910.1053/j.gastro.2003.11.00514762788
     [Google Scholar]
  19. WangF. ButlerT. RabbaniG.H. JonesP.K. The acidosis of cholera. Contributions of hyperproteinemia, lactic acidemia, and hyperphosphatemia to an increased serum anion gap.N. Engl. J. Med.1986315251591159510.1056/NEJM1986121831525063785323
     [Google Scholar]
  20. CiezaJ. SoveroY. EstremadoyroL. DumlerF. Electrolyte disturbances in elderly patients with severe diarrhea due to cholera.J. Am. Soc. Nephrol.1995651463146710.1681/ASN.V6514638589324
     [Google Scholar]
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Cholera Toxin Induces Intestinal Secretion in an Acute Renal Failure Rat Model
Ven and Tox 2, e241121198288 (2022); https://doi.org/10.2174/2666121701666211124152145
/content/journals/vat/10.2174/2666121701666211124152145
/content/journals/vat/10.2174/2666121701666211124152145
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  • Article Type:     Research Article
Keyword(s): acute renal failure; Cholera toxin; electrolytes; intestinal fluid; intestinal loop; secretagogue

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