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Volume 18, Issue 2
  • ISSN: 2772-2708
  • E-ISSN: 2772-2716

Abstract

Background

Cutaneous leishmaniasis (CL) is a parasitic disease with a significant burden in the Old World countries.

Objective

In the current study, some of the primary biochemical properties and IFN-γ inducing epitopes with specific binding capacity to human and mouse MHC alleles were predicted for gp46 antigenic protein.

Methods

Several online servers were used to predict physico-chemical traits, allergenicity, antigenicity, transmembrane domain and signal peptide, subcellular localization, post-translational modifications (PTMs), secondary and tertiary structures, tertiary model refining with validations. Also, IEDB web server was used to predict mouse/human cytotoxic T-lymphocyte (CTL) and helper T-lymphocyte (HTL) epitopes.

Results

The 33.25 kDa protein was stable, hydrophilic, antigenic, while non-allergenic, with enhanced thermotolerance and 45 PTM sites. The secondary structure encompassed a random coil, followed by extended strands and helices. Ramachandran-based analysis of the refined model showed 73.1%, 21.6%, 3.4% and 1.9% of residues in the most favored, additional allowed, generously-allowed and disallowed regions, respectively. Epitope screening demonstrated 4 HTL epitopes against seemingly protective HLA alleles, 5 HTL epitopes against the HLA reference set, 3 human CTL epitopes and a number of mouse MHC-restricted epitopes.

Conclusion

This paper provides insights into the bioinformatics characteristics of the gp46 protein as a promising vaccine candidate.

© 2024 Bentham Science Publishers
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Immunoinformatic Analysis of Leishmania Major gp46 Protein and Potential Targets for Vaccination against Leishmaniasis
Rec Adv Inflamm Allergy Drug Discov 18, 129 (2024); https://doi.org/10.2174/0127722708283588240124095057
/content/journals/raiad/10.2174/0127722708283588240124095057
/content/journals/raiad/10.2174/0127722708283588240124095057
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  • Article Type: Research Article
Keyword(s): gp46; immunogenic; immunogenic epitopes; L. major; leishmania gp46; PSA-2; vaccination

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