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- ISSN: 0929-8665
- E-ISSN: 1875-5305
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One-Step Purification of Functional Light-Harvesting 2 Complex from Rhodobacter sphaeroides
- Source: Protein and Peptide Letters, Volume 17, Issue 4, Apr 2010, p. 444 - 448
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- 01 Apr 2010
Abstract
Most common methods for isolation of the light-harvesting 2 (LH2) complex are time-consuming. In our present work, functional LH2 complex was one-step purified from Rhodobacter sphaeroides by immobilized metal-ion affinity chromatography. The purified LH2 complex exhibited high activity in light absorption and energy transfer. Our present work provides an efficient approach for preparation of functional LH2 complex.
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