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- ISSN: 0929-8665
- E-ISSN: 1875-5305
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Furin Cleavage of Bacterial Expressed Glutathione-S-Transferase-Pro-Transforming Growth Factor β1 Fusion Protein In Vitro
- Source: Protein and Peptide Letters, Volume 17, Issue 4, Apr 2010, p. 416 - 418
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- 01 Apr 2010
Abstract
To investigate the processing of transforming growth factor β1 (TGFβ1) pro-protein by furin protease we expressed a GST-pro-TGFβ1 fusion protein in bacteria. Analysis of the furin digestion pattern revealed the liberation of 12.5 kDa TGFβ1 monomers. There was no evidence for cleavage of an alternative furin site within the pro-protein.
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