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- ISSN: 0929-8665
- E-ISSN: 1875-5305
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Stationary Phase Expression, Purification, and Characterization of XorKI, a Restriction Endonuclease from Xanthomonas oryzae pv. oryzae
- Source: Protein and Peptide Letters, Volume 17, Issue 3, Mar 2010, p. 381 - 385
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- 01 Mar 2010
Abstract
An endonuclease from Xanthomonas oryzae pathovar oryzae KACC 10331, XorKI, was heterologously produced in Escherichia coli by applying the stationary state induction method. The yield was 5.4 mg of XorKI per liter of LB medium. XorKI existed in multiple oligomeric forms as evidenced by gel filtration chromatography. The specific activity of purified XorKI was 323000 units per mg.
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