Comparative Studies on the Aggregation Behavior of HBPs from Human Seminal Plasma by Dynamic Light Scattering

Heparin binding proteins (HBPs) from human seminal plasma (HSP) were obtained by heparin affinity and size exclusion chromatography. The aggregation/disaggregation of HBPs was followed by dynamic light scattering (DLS) in presence of various physiological ligands such as CaCl2, NaCl, EDTA, cholesterol, adenosine, D-glucose and D-fructose. The aggregation pattern of lactoferrin was also analyzed and compared. The study of interactions of HBPs of HSP may contribute to an understanding mechanisms underlying the fertilization process.