Full text loading...
- ISSN: 0929-8665
- E-ISSN: 1875-5305
-
Characterization of Fluoroalcohols-Induced Intermediates of Mucor miehei Lipase at Low pH
- Source: Protein and Peptide Letters, Volume 15, Issue 4, May 2008, p. 346 - 352
-
- 01 May 2008
Abstract
We have previously characterized an acid-unfolded (UA) state of Mucor miehei lipase at pH 2. The effect of 2,2,2-trifluoroethanol (TFE) and 1,1,1,3,3,3-hexafluoroisopropanol (HFIP) resulted in characterization of molten-globule (MG) like states with β-sheet secondary structure at 15% (v/v) TFE and 6% (v/v) HFIP. α-Helical states accumulate at 80% (v/v) TFE and 30% (v/v) HFIP.
© Bentham Science Publishers