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- ISSN: 0929-8665
- E-ISSN: 1875-5305
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Expression and Purification of CB2 for NMR Studies in Micellar Solution
- Source: Protein and Peptide Letters, Volume 14, Issue 10, Oct 2007, p. 1031 - 1037
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- 01 Oct 2007
Abstract
We demonstrate feasibility of biophysical characterization of the peripheral cannabinoid receptor CB2 produced by heterologous expression in E. coli membranes. Recombinant receptor was purified by affinity chromatography, and NMR diffusion experiments performed on CB2 solubilized in dodecylphosphocholine (DPC) micelles. Circular dichroism spectroscopy indicated high α-helical content (49 %) of CB2.
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