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- ISSN: 0929-8665
- E-ISSN: 1875-5305
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Ultrasensitive Staining-Free Protein Detection After PAA Gel Electrophoresis Using Deep UV Fluorescence
- Source: Protein and Peptide Letters, Volume 14, Issue 7, Jul 2007, p. 712 - 715
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- 01 Jul 2007
Abstract
We present the observation of separated protein bands after polyacrylamide (PAA) gel electrophoresis based on the staining-free detection of their ultra violet (UV)-induced fluorescence employing deep UV confocal fluorescence microscopy. Mixtures of the three biological compounds β-Galactosidase (from Escherichia coli), apo-Transferrin (bovine) and bovine serum albumin (BSA) have been separated and a staining free detection limit below 80 pg (7.0x108 molecules) per band has been achieved. This corresponds to ∼270 molecules in the detection volume for confocal microscopy.
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