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- ISSN: 0929-8665
- E-ISSN: 1875-5305
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Expression, Purification and Characterisation of Haemophilus influenzae 3-Isopropylmalate Dehydrogenase (LeuB)
- Source: Protein and Peptide Letters, Volume 14, Issue 8, Aug 2007, p. 822 - 827
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- 01 Aug 2007
Abstract
3-isopropylmalate dehydrogenase (3IPMDH) is the third enzyme in leucine biosynthesis and a promising target for the development of broad-spectrum antibacterial agents. We report here the expression, purification and biochemical characterisation of Haemophilus influenzae 3-isopropylmalate dehydrogenase. The observed enzyme inhibition by the reaction product NADH could represent a regulatory mechanism for 3IPMDH.
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