Full text loading...
- ISSN: 0929-8665
- E-ISSN: 1875-5305
-
High-Level Bacterial Expression and Purification of Apicomplexan Micronemal Proteins for Structural Studies
- Source: Protein and Peptide Letters, Volume 14, Issue 5, May 2007, p. 411 - 415
-
- 01 May 2007
- Previous Article
- Table of Contents
- Next Article
Abstract
The cysteine-rich N-terminal domain of the micronemal adhesive protein MIC1 (MIC1-NT) from Toxoplasma gondii was cloned, expressed in Escherichia coli and purified. MIC1-NT is amenable to structural studies as shown by preliminary NMR and X-ray analysis. Positive results with two further micronemal proteins indicate that our strategy has wider application.
© Bentham Science Publishers