Large Scale Preparation of the Mammalian High Mobility Group Protein A2 for Biophysical Studies

Tengjiao Cui; Suzanne Joynt; Victor Morillo; Maria Baez; Zhichun Hua; Xiaotang Wang; Fenfei Leng

doi:10.2174/092986607779117281

ISSN: 0929-8665
E-ISSN: 1875-5305

Large Scale Preparation of the Mammalian High Mobility Group Protein A2 for Biophysical Studies
Authors: Tengjiao Cui¹, Suzanne Joynt², Victor Morillo³, Maria Baez⁴, Zhichun Hua⁵, Xiaotang Wang⁶ and Fenfei Leng⁷
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¹ Department of Chemistry and Biochemistry, Florida International University, 11200 SW 8th Street, Miami,Florida 33199, USA. ² Department of Chemistry and Biochemistry, Florida International University, 11200 SW 8th Street, Miami,Florida 33199, USA. ³ Department of Chemistry and Biochemistry, Florida International University, 11200 SW 8th Street, Miami,Florida 33199, USA. ⁴ Department of Chemistry and Biochemistry, Florida International University, 11200 SW 8th Street, Miami,Florida 33199, USA. ⁵ Department of Chemistry and Biochemistry, Florida International University, 11200 SW 8th Street, Miami,Florida 33199, USA. ⁶ Department of Chemistry and Biochemistry, Florida International University, 11200 SW 8th Street, Miami,Florida 33199, USA. ⁷ Department of Chemistry and Biochemistry, Florida International University, 11200 SW 8th Street, Miami,Florida 33199, USA.
Source: Protein and Peptide Letters, Volume 14, Issue 1, Jan 2007, p. 87 - 91
DOI: https://doi.org/10.2174/092986607779117281
- Available online: 01 Jan 2007

Abstract

Due to asymmetrical charge distribution of the mammalian high mobility group protein A2 (HMGA2), which makes HMGA2 bind to both cation- and anion-exchange columns, we developed a rapid procedure for purifying HMGA2 in the milligram range. This purification procedure greatly facilitated biophysical studies, which require large amounts of the protein.

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2007-01-01

2025-05-24

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Article Type: Research Article

Keyword(s): differential scanning calorimetry; DNA binding protein; intrinsically unstructured protein; isothermal titration calorimetry; nuclear magnetic resonance (NMR) Spectroscopy; The mammalian high mobility group protein A2 (HMGA2)

Large Scale Preparation of the Mammalian High Mobility Group Protein A2 for Biophysical Studies

Abstract

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