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- ISSN: 0929-8665
- E-ISSN: 1875-5305
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Two-Dimensional Gel Electrophoresis Based Technologies for Potential Biomarkers Identification in Amniotic Fluid: A Simple Model
- Source: Protein and Peptide Letters, Volume 13, Issue 10, Oct 2006, p. 959 - 963
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- 01 Oct 2006
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Abstract
To assess the efficiency of two-dimensional fluorescence difference gel electrophoresis (2D-DIGE) and of twodimensional electrophoresis and ammoniacal silver staining (2D-E), different amounts of soybean trypsin inhibitor and horse myoglobin were added to amniotic fluid. In this model, a minimum of 5 to 10 ng of “artificial” biomarkers was detected.
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