Methodological Improvements on Extraction of Nuclear Proteins and Its Preliminary Analysis During the Maize (Zea mays L.) Endosperm Development

B. S. Ferreira; A. T. Branco; M. A. de Oliveira; M. G. Pereira; G. A. de Souza Filho

doi:10.2174/092986606778777470

ISSN: 0929-8665
E-ISSN: 1875-5305

Methodological Improvements on Extraction of Nuclear Proteins and Its Preliminary Analysis During the Maize (Zea mays L.) Endosperm Development
Authors: B. S. Ferreira¹, A. T. Branco², M. A. de Oliveira³, M. G. Pereira⁴ and G. A. de Souza Filho⁵
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¹ Laboratorio de Biotecnologia,Centro de Biociencias e Biotecnologia, Universidade Estadual do Norte Fluminense Darcy Ribeiro, Av. Alberto Lamego 2000-CEP 28015-602-Campos dos Goytacazes- RJ-Brasil. ² Laboratorio de Biotecnologia,Centro de Biociencias e Biotecnologia, Universidade Estadual do Norte Fluminense Darcy Ribeiro, Av. Alberto Lamego 2000-CEP 28015-602-Campos dos Goytacazes- RJ-Brasil. ³ Laboratorio de Biotecnologia,Centro de Biociencias e Biotecnologia, Universidade Estadual do Norte Fluminense Darcy Ribeiro, Av. Alberto Lamego 2000-CEP 28015-602-Campos dos Goytacazes- RJ-Brasil. ⁴ Laboratorio de Biotecnologia,Centro de Biociencias e Biotecnologia, Universidade Estadual do Norte Fluminense Darcy Ribeiro, Av. Alberto Lamego 2000-CEP 28015-602-Campos dos Goytacazes- RJ-Brasil. ⁵ Laboratorio de Biotecnologia,Centro de Biociencias e Biotecnologia, Universidade Estadual do Norte Fluminense Darcy Ribeiro, Av. Alberto Lamego 2000-CEP 28015-602-Campos dos Goytacazes- RJ-Brasil.
Source: Protein and Peptide Letters, Volume 13, Issue 10, Oct 2006, p. 981 - 984
DOI: https://doi.org/10.2174/092986606778777470
- Available online: 01 Oct 2006

Abstract

A procedure to obtain endosperm protein extracts was standardized. After confirming the enrichment with nuclear proteins by immunodetection, the protein profiles of extracts from different seed development stages were compared by SDS-PAGE that showed the existence of several differentially expressed proteins.

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2006-10-01

2025-06-19

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Article Type: Research Article

Keyword(s): and maize; endosperm development; Nuclear proteins

Methodological Improvements on Extraction of Nuclear Proteins and Its Preliminary Analysis During the Maize (Zea mays L.) Endosperm Development

Abstract

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