Kinetic and Thermodynamic Properties of an Immobilized Glucoamylase from a Mesophilic Fungus, Arachniotus citrinus

Raheela Perveen; Muhammad Hamid Rashid; Muhammad Saleem; Ahmad Mukhtar Khalid; Muhammad Ibrahim Rajoka

doi:10.2174/092986606777790539

ISSN: 0929-8665
E-ISSN: 1875-5305

Kinetic and Thermodynamic Properties of an Immobilized Glucoamylase from a Mesophilic Fungus, Arachniotus citrinus
Authors: Raheela Perveen¹, Muhammad Hamid Rashid², Muhammad Saleem³, Ahmad Mukhtar Khalid⁴ and Muhammad Ibrahim Rajoka⁵
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¹ Industrial Biotechnology Division, National Institute for Biotechnology and Genetic Engineering,P.O. Box 577, Jhang Road, Faisalabad, Pakistan. ² Industrial Biotechnology Division, National Institute for Biotechnology and Genetic Engineering,P.O. Box 577, Jhang Road, Faisalabad, Pakistan. ³ Industrial Biotechnology Division, National Institute for Biotechnology and Genetic Engineering,P.O. Box 577, Jhang Road, Faisalabad, Pakistan. ⁴ Industrial Biotechnology Division, National Institute for Biotechnology and Genetic Engineering,P.O. Box 577, Jhang Road, Faisalabad, Pakistan. ⁵ Industrial Biotechnology Division, National Institute for Biotechnology and Genetic Engineering,P.O. Box 577, Jhang Road, Faisalabad, Pakistan.
Source: Protein and Peptide Letters, Volume 13, Issue 7, Jul 2006, p. 665 - 671
DOI: https://doi.org/10.2174/092986606777790539
- Available online: 01 Jul 2006

Abstract

Purified glucoamylase from Arachniotus citrinus was immobilized on polyacrylamide gel with 70% yield of immobilization. The immobilization improved the pH optima, temperature optima, values of Km, Vmax, and activation energy. Irreversible thermal denaturation studies of soluble and immobilized glucoamylase indicated that immobilization decreased the entropy and enthalpy of deactivation by magnitudes and made the immobilized glucoamylase thermodynamically more stable.

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2006-07-01

2025-05-28

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Article Type: Research Article

Keyword(s): Arachniotus citrinus; enthalpy; entropy; Glucoamylase; thermodynamics

Kinetic and Thermodynamic Properties of an Immobilized Glucoamylase from a Mesophilic Fungus, Arachniotus citrinus

Abstract

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