Expression, Purification, Crystallization and Preliminary Crystallographic Analysis of the Human Intracellular Chloride Channel Protein CLIC4

De-Feng Li; Yun-Feng Li; Qiu-Hua Huang; Ying Zhang; Da-Cheng Wang

doi:10.2174/092986606777145742

ISSN: 0929-8665
E-ISSN: 1875-5305

Expression, Purification, Crystallization and Preliminary Crystallographic Analysis of the Human Intracellular Chloride Channel Protein CLIC4
Authors: De-Feng Li¹, Yun-Feng Li², Qiu-Hua Huang³, Ying Zhang⁴ and Da-Cheng Wang⁵
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¹ Center for Structural and Molecular Biology, Institute of Biophysics, Chinese Academy of Science, Beijing, 100101, People's Republic of China. ² Center for Structural and Molecular Biology, Institute of Biophysics, Chinese Academy of Science, Beijing, 100101, People's Republic of China. ³ Center for Structural and Molecular Biology, Institute of Biophysics, Chinese Academy of Science, Beijing, 100101, People's Republic of China. ⁴ Center for Structural and Molecular Biology, Institute of Biophysics, Chinese Academy of Science, Beijing, 100101, People's Republic of China. ⁵ Center for Structural and Molecular Biology, Institute of Biophysics, Chinese Academy of Science, Beijing, 100101, People's Republic of China.
Source: Protein and Peptide Letters, Volume 13, Issue 6, Jun 2006, p. 641 - 643
DOI: https://doi.org/10.2174/092986606777145742
- Available online: 01 Jun 2006

Abstract

The human chloride intracellular channel protein CLIC4 has been crystallized by the hanging-drop vapourdiffusion technique using trisodium citrate as the precipitant. The best crystals were obtained by the microseeding method. The crystals diffracted to 2.2 Å resolution and were found to belong to space group P121, with unit-cell parameters a = 73.19, b =86.05, c = 73.38 Å, β = 112.99° and three molecule per asymmetric unit.

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2006-06-01

2025-05-23

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Article Type: Research Article

Keyword(s): CLIC4; crystallization; human chloride intracellular channel protein; preliminary crystallographic studies; purification

Expression, Purification, Crystallization and Preliminary Crystallographic Analysis of the Human Intracellular Chloride Channel Protein CLIC4

Abstract

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