Crystallization and Preliminary X-Ray Crystallographic Analysis of Yeast Tyrosyl-tRNA Synthetase Complexed with Its Cognate tRNA

Yoshio Kusakabe; Satoshi Ohno; Nobutada Tanaka; Masashi Nakamura; Masaru Tsunoda; Tomohisa Moriguchi; Norio Asai; Mitsuo Sekine; Takashi Yokogawa; Kazuya Nishikawa; Kazuo T. Nakamura

doi:10.2174/092986606775974465

ISSN: 0929-8665
E-ISSN: 1875-5305

Crystallization and Preliminary X-Ray Crystallographic Analysis of Yeast Tyrosyl-tRNA Synthetase Complexed with Its Cognate tRNA
Authors: Yoshio Kusakabe¹, Satoshi Ohno², Nobutada Tanaka³, Masashi Nakamura⁴, Masaru Tsunoda⁵, Tomohisa Moriguchi⁶, Norio Asai⁷, Mitsuo Sekine⁸, Takashi Yokogawa⁹, Kazuya Nishikawa¹⁰ and Kazuo T. Nakamura¹¹
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¹ School of Pharmaceutical Sciences, Showa University, Tokyo 142-8555, Japan. ² School of Pharmaceutical Sciences, Showa University, Tokyo 142-8555, Japan. ³ School of Pharmaceutical Sciences, Showa University, Tokyo 142-8555, Japan. ⁴ School of Pharmaceutical Sciences, Showa University, Tokyo 142-8555, Japan. ⁵ School of Pharmaceutical Sciences, Showa University, Tokyo 142-8555, Japan. ⁶ School of Pharmaceutical Sciences, Showa University, Tokyo 142-8555, Japan. ⁷ School of Pharmaceutical Sciences, Showa University, Tokyo 142-8555, Japan. ⁸ School of Pharmaceutical Sciences, Showa University, Tokyo 142-8555, Japan. ⁹ School of Pharmaceutical Sciences, Showa University, Tokyo 142-8555, Japan. ¹⁰ School of Pharmaceutical Sciences, Showa University, Tokyo 142-8555, Japan. ¹¹ School of Pharmaceutical Sciences, Showa University, Tokyo 142-8555, Japan.
Source: Protein and Peptide Letters, Volume 13, Issue 4, Apr 2006, p. 417 - 419
DOI: https://doi.org/10.2174/092986606775974465
- Available online: 01 Apr 2006

Abstract

Yeast tyrosyl-tRNA synthetase (yTyrRS) has been crystallized by the vapor diffusion method in the presence of its cognate tRNATyr. The crystals belong to a tetragonal space group P41212 with cell dimensions of a = b = 63.85 Å, and c = 330.3 Å. The asymmetric unit contains one molecule each of yTyrRS and tRNATyr (one-half of a 2:2 complex). Xray diffraction data have been collected up to 2.5 Å resolution.

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2006-04-01

2025-05-21

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Article Type: Research Article

Keyword(s): aminoacyl-tRNA synthetase; crystallization; tRNA; TyrRS

Crystallization and Preliminary X-Ray Crystallographic Analysis of Yeast Tyrosyl-tRNA Synthetase Complexed with Its Cognate tRNA

Abstract

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